Fukushima Y, Lewis M J, Monken C, Komuro K, Kusagawa S, Sato H, Takebe Y, Yamazaki S, Nguyen T H, Hoang A, Hoang T L, Honda M, Hall W W
Department of Medical Microbiology, University College Dublin, Ireland.
AIDS Res Hum Retroviruses. 1998 Apr 10;14(6):537-40. doi: 10.1089/aid.1998.14.537.
Previous serological studies have demonstrated that some 60% of intravenous drug abusers (IVDAs) in urban areas of the former South Vietnam are infected with HTLV-II. In the present report we have attempted to characterize the viruses using restriction fragment length polymorphism (RFLP) and nucleotide sequence analysis of the provirus long terminal repeat (LTR) region. RFLP analysis of nine samples demonstrated that all were infected with the HTLV-IIb subtype. The HTLV-IIa subtype was not detected. Phylogenetic analysis of the nucleotide sequences demonstrated that the viruses clustered closely with HTLV-IIb isolates present in IVDAs from the New York City area. The present molecular analysis together with the previously reported absence of HTLV-II infection in North Vietnam supports the view that HTLV-II may have been introduced from the United States to this part of Asia by military personnel during the Vietnam conflict.
以往的血清学研究表明，在前南越城市地区，约60%的静脉注射吸毒者（IVDA）感染了HTLV-II。在本报告中，我们试图利用限制片段长度多态性（RFLP）和前病毒长末端重复序列（LTR）区域的核苷酸序列分析来鉴定这些病毒。对9个样本的RFLP分析表明，所有样本均感染了HTLV-IIb亚型。未检测到HTLV-IIa亚型。核苷酸序列的系统发育分析表明，这些病毒与纽约市地区IVDA中存在的HTLV-IIb分离株密切聚集。目前的分子分析以及先前报道的北越不存在HTLV-II感染的情况支持了这样一种观点，即HTLV-II可能是在越南冲突期间由军事人员从美国引入亚洲这一地区的。
