Ash1是一种子细胞特异性蛋白，是酿酒酵母假菌丝生长所必需的。
Ash1, a daughter cell-specific protein, is required for pseudohyphal growth of Saccharomyces cerevisiae.
作者信息
Chandarlapaty S, Errede B
机构信息
Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill 27599-7260, USA.
出版信息
Mol Cell Biol. 1998 May;18(5):2884-91. doi: 10.1128/MCB.18.5.2884.
Abstract
Ash1 (for asymmetric synthesis of HO) was first uncovered in genetic screens that revealed its role in mating-type switching. Ash1 prevents HO expression in daughter cells. Because Ash1 has a zinc finger-like domain related to that of the GATA family of transcription factors, it presumably acts by repressing HO transcription. Nonswitching diploid cells also express Ash1, suggesting it could have functions in addition to regulation of HO expression. We show here that Ash1 has an essential function for pseudohyphal growth. Our epistasis analyses are consistent with the deduction that Ash1 acts separately from the mitogen-activated protein kinase cascade and Ste12. Similarly to the case in yeast form cells, Ash1 is asymmetrically localized to the nuclei of daughter cells during pseudohyphal growth. This asymmetric localization reveals that there is a previously unsuspected daughter cell-specific function necessary for pseudohyphal growth.
摘要
Ash1（用于HO的不对称合成）最初是在遗传筛选中被发现的，这些筛选揭示了它在交配型转换中的作用。Ash1可阻止子细胞中HO的表达。由于Ash1具有与GATA转录因子家族相关的锌指样结构域，推测它可能通过抑制HO转录来发挥作用。不进行转换的二倍体细胞也表达Ash1，这表明它可能除了调节HO表达外还具有其他功能。我们在此表明，Ash1对假菌丝生长具有重要作用。我们的上位性分析与Ash1与丝裂原活化蛋白激酶级联反应和Ste12分开起作用的推断一致。与酵母形态细胞中的情况类似，在假菌丝生长过程中，Ash1不对称地定位于子细胞核中。这种不对称定位表明，假菌丝生长需要一种以前未被怀疑的子细胞特异性功能。
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