Marschner I, Erhardt F W, Scriba P C
J Clin Chem Clin Biochem. 1976 Jul;14(7):345-51.
This paper deals with a cooperative survey on the radioimmunoassay of thyrotropin in human serum, which was organized by the Thyroid Group of the German Society for Endocrinology. Eighteen serum specimens were to be measured in triplicates. Among the latter were 10 specimens of a hTSH standard curve, prepared in low hTSH serum, which contained approximately 1 mU/l. One of these pool sera was divided in half, and one half was enriched with 10 kU/l human chorionic gonadotropin to test for cross reactivity. One specimen las for the measurement of the non-specific binding. The count rates and the dose levels of the standard curve of each participating laboratory (N = 32), normally performed in albumin-buffer, and the count rates of the 18 unknowns were requested as results. THE CALCULATION OF THE STANDARD CURVES AND THE RECOVERy curves, the evaluation of the unknowns and the statistics were performed by spline functions, using a 48 kb FORTRAN-program on a Siemens 404/3 computer. The results demonstrated that the interlaboratory precision of 128% and the recovery rates between 50 and 926% are very unsatisfactory. However, the coefficient of variation was lowered to 31%, when the controls were read from the recovery curves, as included in the samples. In addition to this improvement of the interlaboratory precision and of the accuracy, this study showed, that the sensitivity was markedly improved by using delayed addition of tracer.
本文论述了由德国内分泌学会甲状腺组组织的一项关于人血清促甲状腺激素放射免疫测定的协作研究。18份血清标本需进行三次重复测定。其中有10份在低促甲状腺激素血清中制备的人促甲状腺激素标准曲线标本，其浓度约为1 mU/l。这些混合血清中的一份被分成两半，其中一半加入10 kU/l人绒毛膜促性腺激素以检测交叉反应性。有一份标本用于非特异性结合的测定。要求各参与实验室（N = 32）通常在白蛋白缓冲液中进行的标准曲线的计数率和剂量水平，以及18份未知标本的计数率作为结果。标准曲线和回收率曲线的计算、未知标本的评估以及统计学分析通过样条函数进行，使用西门子404/3计算机上一个48 kb的FORTRAN程序。结果表明，实验室间精密度为128%，回收率在50%至926%之间，非常不理想。然而，当从样本中包含的回收率曲线读取对照时，变异系数降至31%。除了实验室间精密度和准确性的这种提高外，本研究还表明，通过采用延迟加入示踪剂，灵敏度有显著提高。
