Salonen E M, Parren P W, Graus Y F, Lundkvist A, Fisicaro P, Vapalahti O, Kallio-Kokko H, Vaheri A, Burton D R
Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037, USA.
J Gen Virol. 1998 Apr;79 ( Pt 4):659-65. doi: 10.1099/0022-1317-79-4-659.
A panel of seven human monoclonal Fabs against Puumala virus (PUU) nucleocapsid protein (N) was obtained by panning an antibody phage-display library prepared from the spleen of a PUU-immune individual. Three antibodies reacted in immunoblotting and cross-reacted strongly with Tula and Sin Nombre virus recombinant N proteins. These antibodies mapped to the amino terminus of the N protein. One PUU glycoprotein 2 (G2)-specific Fab obtained against a novel epitope (G2c) cross-reacted with Khabarovsk virus but not with the other hantavirus serotypes. An N protein-specific Fab was successfully used as capture antibody to detect PUU-specific serum IgG and IgM antibodies in an enzyme immunoassay. The result demonstrates the usefulness of recombinant human Fabs as potential diagnostic tools.
通过淘选从感染普马拉病毒（PUU）个体脾脏制备的抗体噬菌体展示文库，获得了一组针对普马拉病毒核衣壳蛋白（N）的七种人源单克隆Fab片段。三种抗体在免疫印迹中发生反应，并与图拉病毒和辛诺柏病毒重组N蛋白发生强烈交叉反应。这些抗体定位于N蛋白的氨基末端。一种针对新型表位（G2c）获得的普马拉病毒糖蛋白2（G2）特异性Fab片段与哈巴罗夫斯克病毒发生交叉反应，但与其他汉坦病毒血清型无交叉反应。一种N蛋白特异性Fab片段成功用作捕获抗体，在酶免疫测定中检测普马拉病毒特异性血清IgG和IgM抗体。结果证明重组人源Fab片段作为潜在诊断工具的实用性。
