Vapalahti O, Lundkvist A, Fedorov V, Conroy C J, Hirvonen S, Plyusnina A, Nemirov K, Fredga K, Cook J A, Niemimaa J, Kaikusalo A, Henttonen H, Vaheri A, Plyusnin A
Department of Virology, Haartman Institute, FIN-00014 University of Helsinki, Finland.
J Virol. 1999 Jul;73(7):5586-92. doi: 10.1128/JVI.73.7.5586-5592.1999.
A novel hantavirus, first detected in Siberian lemmings (Lemmus sibiricus) collected near the Topografov River in the Taymyr Peninsula, Siberia (A. Plyusnin et al., Lancet 347:1835-1836, 1996), was isolated in Vero E6 cells and in laboratory-bred Norwegian lemmings (Lemmus lemmus). The virus, named Topografov virus (TOP), was most closely related to Khabarovsk virus (KBR) and Puumala viruses (PUU). In a cross focus reduction neutralization test, anti-TOP Lemmus antisera showed titers at least fourfold higher with TOP than with other hantaviruses; however, a rabbit anti-KBR antiserum neutralized TOP and KBR at the same titer. The TOP M segment showed 77% nucleotide and 88% amino acid identity with KBR and 76% nucleotide and 82% amino acid identity with PUU. However, the homology between TOP and the KBR S segment was disproportionately higher: 88% at the nucleotide level and 96% at the amino acid level. The 3' noncoding regions of KBR and the TOP S and M segments were alignable except for 113- and 58-nucleotide deletions in KBR. The phylogenetic relationships of TOP, KBR, and PUU and their respective rodent carriers suggest that an exceptional host switch took place during the evolution of these viruses; while TOP and KBR are monophyletic, the respective rodent host species are only distantly related.
一种新型汉坦病毒，最初是在西伯利亚泰梅尔半岛托普ografov河附近捕获的西伯利亚旅鼠（Lemmus sibiricus）中检测到的（A. Plyusnin等人，《柳叶刀》347:1835 - 1836，1996），该病毒在Vero E6细胞和实验室培育的挪威旅鼠（Lemmus lemmus）中分离得到。这种病毒被命名为托普ografov病毒（TOP），与哈巴罗夫斯克病毒（KBR）和普马拉病毒（PUU）关系最为密切。在交叉空斑减少中和试验中，抗TOP旅鼠抗血清对TOP的效价比对其他汉坦病毒至少高四倍；然而，兔抗KBR抗血清对TOP和KBR的中和效价相同。TOP的M片段与KBR的核苷酸同一性为77%，氨基酸同一性为88%，与PUU的核苷酸同一性为76%，氨基酸同一性为82%。然而，TOP与KBR的S片段之间的同源性异常高：核苷酸水平为88%，氨基酸水平为96%。除了KBR中有113和58个核苷酸的缺失外，KBR以及TOP的S和M片段的3'非编码区是可比对的。TOP、KBR和PUU及其各自啮齿动物宿主的系统发育关系表明，在这些病毒的进化过程中发生了一次特殊的宿主转换；虽然TOP和KBR是单系的，但各自的啮齿动物宿主物种关系较远。
