Matsumoto T, Tateda K, Furuya N, Miyazaki S, Ohno A, Ishii Y, Hirakata Y, Yamaguchi K
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
J Med Microbiol. 1998 Apr;47(4):303-8. doi: 10.1099/00222615-47-4-303.
The protective efficacies of vaccines prepared from Pseudomonas aeruginosa alkaline protease, elastase and exotoxin A toxoids against gut-derived P. aeruginosa sepsis in mice were evaluated. Specific pathogen-free mice given P. aeruginosa strain D4 orally followed by cyclophosphamide (to promote translocation across the gut wall) died of bacteraemia. Mice immunised with one of the three individual toxoid vaccines were not significantly protected when compared to control mice immunised with bovine serum albumin. Combined immunisation with alkaline protease and elastase toxoids likewise showed no significant protective activity. However, combined immunisation with alkaline protease and exotoxin A toxoids significantly increased the survival rate, which reached 60% (compared with a 7.1% survival rate in the control group). These results show that alkaline protease and exotoxin A play important roles as pathogenic factors in gut-derived sepsis and that a combination of the two exoenzyme toxoids represents a logical candidate for vaccination against P. aeruginosa sepsis.
评估了由铜绿假单胞菌碱性蛋白酶、弹性蛋白酶和外毒素A类毒素制备的疫苗对小鼠肠道源性铜绿假单胞菌败血症的保护效力。给无特定病原体小鼠口服铜绿假单胞菌D4菌株，随后给予环磷酰胺（以促进细菌穿过肠壁移位），小鼠死于菌血症。与用牛血清白蛋白免疫的对照小鼠相比，用三种单独类毒素疫苗之一免疫的小鼠没有得到显著保护。碱性蛋白酶和弹性蛋白酶类毒素联合免疫同样没有显示出显著的保护活性。然而，碱性蛋白酶和外毒素A类毒素联合免疫显著提高了存活率，达到60%（对照组存活率为7.1%）。这些结果表明，碱性蛋白酶和外毒素A作为肠道源性败血症的致病因素发挥重要作用，并且这两种外酶类毒素的组合是预防铜绿假单胞菌败血症疫苗的合理候选物。
