Hills B A, Monds M K
Paediatric Respiratory Research Centre, Mater Children's Hospital, Brisbane, Queensland, Australia.
Br J Rheumatol. 1998 Feb;37(2):137-42. doi: 10.1093/oxfordjournals.rheumatology.a031463.
Bovine articular cartilage and synovial fluid (SF) were co-incubated with one of three enzymes selected to destroy each of the three major contenders for the active ingredient imparting such remarkable load-bearing lubrication to the normal joint. Destroying hyaluronic acid (HA), alias hyaluronan, with hyaluronidase, both frictional and wear tests displayed no significant change in accordance with most previous studies of SF alone. Destroying surface-active phospholipid (SAPL) with phospholipase A2, there was a highly significant dose-dependent compromise of lubrication as recorded on both tests. Trypsin produced a somewhat surprising result in that lubrication of the cartilage actually improved. This result can be interpreted as indicating that lubricin is not the lubricant per se, but, as a water-soluble, macromolecular, proteinaceous carrier for phospholipid, its destruction caused more SAPL to be deposited as the true load-bearing lubricant. These results are discussed in the context that SAPL, lubricin and HA each have specific roles in a comprehensive lubrication system.
将牛关节软骨和滑液（SF）与三种酶之一共同孵育，这三种酶被选定用于破坏正常关节中赋予卓越承重润滑性能的三种主要活性成分中的每一种。用透明质酸酶破坏透明质酸（HA）（又称透明质聚糖）后，摩擦和磨损测试均显示无显著变化，这与之前大多数仅针对滑液的研究结果一致。用磷脂酶A2破坏表面活性磷脂（SAPL）后，两项测试均记录到润滑性能出现高度显著的剂量依赖性损害。胰蛋白酶产生了一个 somewhat surprising result（此处原文有误，应改为“somewhat surprising result”，意为“有点令人惊讶的结果”），即软骨的润滑性能实际上得到了改善。这一结果可以解释为表明润滑素本身并非润滑剂，而是作为磷脂的水溶性、大分子、蛋白质载体，其破坏导致更多的SAPL作为真正的承重润滑剂沉积下来。本文在SAPL、润滑素和HA在综合润滑系统中各自具有特定作用的背景下对这些结果进行了讨论。
