Sun Yulong, Chen Meng-Yi, Zhao Chunfeng, An Kai-Nan, Amadio Peter C
Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, 200 First Street SW, Rochester, Minnesota 55905, USA.
J Orthop Res. 2008 Sep;26(9):1225-9. doi: 10.1002/jor.20624.
Although the flexor digitorum profundus (FDP) tendon's gliding resistance is low, the lubrication mechanism that enables this is unclear. The principal lubricants in joints, such as hyaluronic acid, phospholipids, and lubricin, a lubricating glycoprotein, are known to be present in the canine tendon. In this study, we assessed the effect of these lubricants in the tendon by measuring gliding resistance before and after their removal. Canine FDP tendons were treated with hyaluronidase, phospholipase, lipid solvent, and/or trypsin. The gliding resistance of FDP tendons significantly increased after all treatments (p < 0.05). The largest effect on gliding resistance was observed after trypsin digestion. Scanning electron microscopy and immunostaining for hyaluronic acid and lubricin were used to qualitatively assess the tendon surface after treatments. The trypsin digestion produced the most irregular surface, with many exposed collagen fibers. The results of this study suggest that phospholipids, hyaluronic acid, and protein components are all involved in maintaining the low gliding resistance of the FDP tendon.
尽管指深屈肌（FDP）肌腱的滑动阻力较低，但实现这一现象的润滑机制尚不清楚。关节中的主要润滑剂，如透明质酸、磷脂和润滑素（一种润滑糖蛋白），已知存在于犬类肌腱中。在本研究中，我们通过测量这些润滑剂去除前后的滑动阻力来评估它们在肌腱中的作用。犬类FDP肌腱用透明质酸酶、磷脂酶、脂质溶剂和/或胰蛋白酶处理。所有处理后，FDP肌腱的滑动阻力均显著增加（p < 0.05）。胰蛋白酶消化后观察到对滑动阻力的影响最大。使用扫描电子显微镜以及对透明质酸和润滑素进行免疫染色，对处理后的肌腱表面进行定性评估。胰蛋白酶消化产生的表面最不规则，有许多暴露的胶原纤维。本研究结果表明，磷脂、透明质酸和蛋白质成分均参与维持FDP肌腱的低滑动阻力。
