用于检测慢性恰加斯病患者血样中克氏锥虫的聚合酶链反应程序。

Polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients.

作者信息

Carriazo C S, Sembaj A, Aguerri A M, Requena J M, Alonso C, Búa J, Ruiz A, Segura E, Barral J M

机构信息

Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.

出版信息

Diagn Microbiol Infect Dis. 1998 Mar;30(3):183-6. doi: 10.1016/s0732-8893(97)00238-1.

DOI:10.1016/s0732-8893(97)00238-1

PMID:9572024

Abstract

The feasibility of DNA amplification by the polymerase chain reaction for specific detection of Trypanosoma cruzi in human blood was investigated. We have used primers flanking a 220-bp fragment of highly repetitive elements, the E13 element, in T. cruzi nuclear DNA. Only polymerase chain reaction products from blood samples of chronic chagasic patients showed several amplified fragments in 1.6% agarose gels stained with ethidium bromide. Southern blot analysis demonstrated that the 220-bp amplified fragment is specific for T. cruzi DNA and very useful to detect the presence of the parasite in blood from chronic chagasic patients.

摘要

研究了通过聚合酶链反应进行DNA扩增以特异性检测人血液中克氏锥虫的可行性。我们使用了位于克氏锥虫核DNA中高度重复元件E13元件的220 bp片段两侧的引物。只有慢性恰加斯病患者血液样本的聚合酶链反应产物在经溴化乙锭染色的1.6%琼脂糖凝胶中显示出几个扩增片段。Southern印迹分析表明，220 bp的扩增片段对克氏锥虫DNA具有特异性，对于检测慢性恰加斯病患者血液中寄生虫的存在非常有用。