木腐真菌（鬼伞科）漆酶和过氧化物酶的特性研究
Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae).
作者信息
Heinzkill M, Bech L, Halkier T, Schneider P, Anke T
机构信息
Department of Biotechnology, University of Kaiserslautern, Germany.
出版信息
Appl Environ Microbiol. 1998 May;64(5):1601-6. doi: 10.1128/AEM.64.5.1601-1606.1998.
Abstract
Panaeolus sphinctrinus, Panaeolus papilionaceus, and Coprinus friesii are described as producers of ligninolytic enzymes. P. papilionaceus and P. sphinctrinus both produced a laccase. In addition, P. sphinctrinus produced a manganese peroxidase. C. friesii secreted a laccase and two peroxidases similar to the peroxidase of Coprinus cinereus. The purified laccases and peroxidases were characterized by broad substrate specificities, significant enzyme activities at alkaline pH values, and remarkably high pH optima. The two peroxidases of C. friesii remained active at pH 7.0 and 60 degrees C for up to 60 min of incubation. The peroxidases were inhibited by sodium azide and ethylene glycol-bis (beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), whereas the laccases were inhibited by sodium azide and N,N-diethyldithiocarbamic acid. As determined by native polyacrylamide gel electrophoresis and isoelectric focusing, all three fungi produced laccase isoenzymes.
摘要
皱盖球盖菇、蝶形球盖菇和弗氏鬼伞被描述为木质素分解酶的产生菌。蝶形球盖菇和皱盖球盖菇均产生一种漆酶。此外，皱盖球盖菇还产生一种锰过氧化物酶。弗氏鬼伞分泌一种漆酶和两种类似于灰盖鬼伞过氧化物酶的过氧化物酶。纯化后的漆酶和过氧化物酶具有广泛的底物特异性、在碱性pH值下具有显著的酶活性以及非常高的最适pH值。弗氏鬼伞的两种过氧化物酶在pH 7.0和60℃下孵育长达60分钟仍保持活性。过氧化物酶受到叠氮化钠和乙二醇双（β-氨基乙基醚）-N,N,N',N'-四乙酸（EGTA）的抑制，而漆酶受到叠氮化钠和N,N-二乙基二硫代氨基甲酸盐的抑制。通过非变性聚丙烯酰胺凝胶电泳和等电聚焦测定，所有三种真菌均产生漆酶同工酶。
相似文献
1
Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae).
Appl Environ Microbiol. 1998 May;64(5):1601-6. doi: 10.1128/AEM.64.5.1601-1606.1998.
2
Production of ligninolytic enzymes and synthetic lignin mineralization by the bird's nest fungus Cyathus stercoreus.
Appl Microbiol Biotechnol. 1999 Nov;52(5):689-97. doi: 10.1007/s002530051580.
3
Isoenzymes of manganese-dependent peroxidase and laccase produced by the lignin-degrading basidiomycete Ceriporiopsis subvermispora.
Microbiology (Reading). 1994 Oct;140 ( Pt 10):2691-8. doi: 10.1099/00221287-140-10-2691.
4
Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197.
Appl Environ Microbiol. 1997 Jul;63(7):2637-46. doi: 10.1128/aem.63.7.2637-2646.1997.
5
Purification and characterization of laccases from the white-rot basidiomycete Dichomitus squalens.
Arch Biochem Biophys. 1998 May 15;353(2):349-55. doi: 10.1006/abbi.1998.0625.
6
Characterization of a novel laccase produced by the wood-rotting fungus Phellinus ribis.
Arch Biochem Biophys. 2001 Aug 15;392(2):279-86. doi: 10.1006/abbi.2001.2459.
7
Purification and characterization of two laccase isoenzymes from a ligninolytic fungus Trametes gallica.
Prep Biochem Biotechnol. 2004 May;34(2):179-94. doi: 10.1081/PB-120030876.
8
Isolation and Purification of enzymes from ligninolytic complex of the basidial fungus Trametes pubescens (Schumach.) Pilát and study of their properties.
Biochemistry (Mosc). 2005 Nov;70(11):1274-9. doi: 10.1007/s10541-005-0259-0.
9
Industrial dye decolorization by laccases from ligninolytic fungi.
Curr Microbiol. 1999 Jan;38(1):27-32. doi: 10.1007/pl00006767.
10
Laccase and other ligninolytic enzyme activities of selected strains of Trametes spp. from different localities and substrates.
Folia Microbiol (Praha). 2003;48(3):413-6. doi: 10.1007/BF02931377.
引用本文的文献
1
Production, Purification and Immobilization of Laccase from HBB 7328 for its Role in Decolorization of Textile Dyes.
Indian J Microbiol. 2024 Dec;64(4):1870-1883. doi: 10.1007/s12088-024-01260-3. Epub 2024 Apr 1.
2
Unusual Oligomeric Laccase-like Oxidases from Ascomycete VKM F-3561 Polymerizing Phenylpropanoids and Phenolic Compounds under Neutral Environmental Conditions.
Microorganisms. 2023 Nov 3;11(11):2698. doi: 10.3390/microorganisms11112698.
3
Bioremoval and Detoxification of Anthracene by a Halophilic Laccase from .
Iran J Biotechnol. 2022 Apr 1;20(2):e3058. doi: 10.30498/ijb.2022.287500.3058. eCollection 2022 Apr.
4
Immobilization of a Broad Range of Polypeptides on the Frustule of the Diatom .
Appl Environ Microbiol. 2022 Nov 8;88(21):e0115322. doi: 10.1128/aem.01153-22. Epub 2022 Oct 13.
5
Bioactives and Extracellular Enzymes Obtained from Fermented Macrofungi Cultivated in Cotton and Seed Cakes.
Microorganisms. 2022 Aug 19;10(8):1670. doi: 10.3390/microorganisms10081670.
6
Genomic analysis uncovers laccase-coding genes and biosynthetic gene clusters encoding antimicrobial compounds in laccase-producing Acinetobacter baumannii.
Sci Rep. 2022 Jul 13;12(1):11932. doi: 10.1038/s41598-022-16122-5.
7
Biochemical and kinetic characterization of laccase and manganese peroxidase from novel strains and their application in bioethanol production.
RSC Adv. 2018 Apr 20;8(27):15044-15055. doi: 10.1039/c8ra01204k. eCollection 2018 Apr 18.
8
Fabrication of a New, Low-Cost, and Environment-Friendly Laccase-Based Biosensor by Electrospray Immobilization with Unprecedented Reuse and Storage Performances.
ACS Sustain Chem Eng. 2022 Feb 7;10(5):1888-1898. doi: 10.1021/acssuschemeng.1c07604. Epub 2022 Jan 24.
9
Engineering the Catalytic Properties of Two-Domain Laccase from Ac-993.
Int J Mol Sci. 2021 Dec 22;23(1):65. doi: 10.3390/ijms23010065.
10
Deletion of the Gene, Involved in the Cell Cycle, Affects Respiration and Pseudohyphal Differentiation in Saccharomyces cerevisiae.
Microbiol Spectr. 2021 Sep 3;9(1):e0008821. doi: 10.1128/Spectrum.00088-21. Epub 2021 Aug 4.
本文引用的文献
1
Roles of Lignin Peroxidase and Manganese Peroxidase from Phanerochaete chrysosporium in the Decolorization of Olive Mill Wastewaters.
Appl Environ Microbiol. 1995 Mar;61(3):1098-103. doi: 10.1128/aem.61.3.1098-1103.1995.
2
Purification, characterization, molecular cloning, and expression of two laccase genes from the white rot basidiomycete Trametes villosa.
Appl Environ Microbiol. 1996 Mar;62(3):834-41. doi: 10.1128/aem.62.3.834-841.1996.
3
The identification and characterization of four laccases from the plant pathogenic fungus Rhizoctonia solani.
Curr Genet. 1996 Mar;29(4):395-403. doi: 10.1007/BF02208621.
4
Isozymes of lignin peroxidase and manganese(II) peroxidase from the white-rot basidiomycete Trametes versicolor. I. Isolation of enzyme forms and characterization of physical and catalytic properties.
Arch Biochem Biophys. 1993 Jan;300(1):49-56. doi: 10.1006/abbi.1993.1007.
5
Crystal structure of the fungal peroxidase from Arthromyces ramosus at 1.9 A resolution. Structural comparisons with the lignin and cytochrome c peroxidases.
J Mol Biol. 1994 Jan 7;235(1):331-44. doi: 10.1016/s0022-2836(05)80037-3.
6
Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Microbiol Rev. 1993 Sep;57(3):605-22. doi: 10.1128/mr.57.3.605-622.1993.
7
Oxidation-reduction properties of compounds I and II of Arthromyces ramosus peroxidase.
Biochemistry. 1994 May 10;33(18):5647-52. doi: 10.1021/bi00184a038.
8
Isoenzymes of manganese-dependent peroxidase and laccase produced by the lignin-degrading basidiomycete Ceriporiopsis subvermispora.
Microbiology (Reading). 1994 Oct;140 ( Pt 10):2691-8. doi: 10.1099/00221287-140-10-2691.
9
Properties of laccase isoenzymes produced by the basidiomycete Ceriporiopsis subvermispora.
Biotechnol Appl Biochem. 1995 Jun;21(3):323-33.
10
Laccase component of the Ceriporiopsis subvermispora lignin-degrading system.
Appl Environ Microbiol. 1995 Mar;61(3):872-6. doi: 10.1128/aem.61.3.872-876.1995.
Zotero 插件