Bioactives and Extracellular Enzymes Obtained from Fermented Macrofungi Cultivated in Cotton and Seed Cakes.

This work focused on obtaining fermented oil cake (cotton or ) via macrofungi growth with potential characteristics for animal feed formulations, such as the presence of extracellular enzymes, bioactive (ergosterol and antioxidants), and detoxification of antinutritional compounds. The concentration of phorbol esters was reduced by four macrofungi in seed cake (JSC) to non-toxic levels. At least two macrofungi efficiently degraded free gossypol in cottonseed cake (CSC). Fermentation with sp. INPA1646 and sp. INPA1696 resulted in increased ergosterol concentrations, antioxidant activity reduction, and high activity of laccases and proteases. Bromatological analysis indicated high crude protein concentrations, with partial solubilization by fungal proteases. Fermented products from sp. and sp. in JSC or CSC can be considered important biological inputs for monogastric and polygastric animal feed.