Rohr U, Weber S, Michel R, Selenka F, Wilhelm M
Institut für Hygiene und Mikrobiologie, Ruhr-Universität Bochum, Germany.
Appl Environ Microbiol. 1998 May;64(5):1822-4. doi: 10.1128/AEM.64.5.1822-1824.1998.
Legionella-contaminated hot water systems and moist sanitary areas in six hospitals were sampled for amoebae by following a standardized collection protocol. Genus identifications and temperature tolerance determinations were made. Amoebae identified as Hartmannella vermiformis (65%), Echinamoebae spp. (15%), Saccamoebae spp. (12%), and Vahlkampfia spp. (9%) were detected in 29 of 56 (52%) hot water samples. Twenty-three of 49 (47%) swabs obtained from moist areas were amoeba positive. The following genera were identified: Acanthamoeba (22%), Naegleria (22%), Vahlkampfia (20%), Hartmannella (15%), and Vanella (7%). The temperature tolerance of amoebae from hot water systems was strikingly different from that of amoebae from moist areas. At 44 degrees C on agar, 59% of amoebic isolates sampled from hot water systems showed growth. The corresponding value for isolates from moist areas was only 17%. Six Acanthamoeba isolates from the moist areas were considered potential pathogens. Four Hartmannella and two Saccamoeba isolates from hot water could be cultured at 53 degrees C.
按照标准化采集方案,对6家医院中受军团菌污染的热水系统和潮湿卫生区域进行采样以检测变形虫。进行了属的鉴定和温度耐受性测定。在56份热水样本中的29份(52%)中检测到了被鉴定为蠕虫哈曼变形虫(65%)、棘变形虫属(15%)、囊变形虫属(12%)和瓦氏变形虫属(9%)的变形虫。从潮湿区域采集的49份拭子中有23份(47%)变形虫呈阳性。鉴定出以下属:棘阿米巴属(22%)、耐格里属(22%)、瓦氏变形虫属(20%)、哈曼变形虫属(15%)和瓦内拉属(7%)。热水系统中变形虫的温度耐受性与潮湿区域变形虫的温度耐受性显著不同。在琼脂上44摄氏度时,从热水系统中采样的变形虫分离株中有59%显示生长。来自潮湿区域分离株的相应值仅为17%。来自潮湿区域的6株棘阿米巴分离株被认为是潜在病原体。来自热水的4株哈曼变形虫和2株囊变形虫分离株可在53摄氏度下培养。