红色丙二酸单胞菌的一种Na⁺依赖性丙二酸转运蛋白的鉴定及其对两个独立基因的依赖性。
Identification of an Na+-dependent malonate transporter of Malonomonas rubra and its dependence on two separate genes.
作者信息
Schaffitzel C, Berg M, Dimroth P, Pos K M
机构信息
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
出版信息
J Bacteriol. 1998 May;180(10):2689-93. doi: 10.1128/JB.180.10.2689-2693.1998.
Abstract
Two membrane proteins encoded by the malonate fermentation gene cluster of Malonomonas rubra, MadL and MadM, have been synthesized in Escherichia coli. MadL and MadM were shown to function together as a malonate transport system, whereas each protein alone was unable to catalyze malonate transport. Malonate transport by MadLM is Na+ dependent, and imposition of a DeltapNa+ markedly enhanced the rate of malonate uptake. The kinetics of malonate uptake into E. coli BL21(DE3) cells synthesizing MadLM at different pH values indicated that Hmalonate- is the transported malonate species. The stimulation of malonate uptake by Na+ ions showed Michaelis-Menten kinetics, and a Km for Na+ of 1.2 mM was determined. These results suggest that MadLM is an electroneutral Na+/Hmalonate- symporter and that it is dependent on two separate genes.
摘要
红丙二酸单胞菌丙二酸发酵基因簇编码的两种膜蛋白MadL和MadM已在大肠杆菌中合成。MadL和MadM被证明可共同作为丙二酸转运系统发挥作用，而单独的每种蛋白质都无法催化丙二酸转运。MadLM介导的丙二酸转运依赖于Na⁺，施加跨膜Na⁺梯度可显著提高丙二酸摄取速率。在不同pH值下，丙二酸摄取到合成MadLM的大肠杆菌BL21(DE3)细胞中的动力学表明，转运的丙二酸形式是Hmalonate⁻。Na⁺离子对丙二酸摄取的刺激呈现米氏动力学，测定出Na⁺的Km值为1.2 mM。这些结果表明，MadLM是一种电中性的Na⁺/Hmalonate⁻同向转运体，并且它依赖于两个独立的基因。
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