金黄色葡萄球菌的青霉素结合蛋白1对生长至关重要。
Penicillin-binding protein 1 of Staphylococcus aureus is essential for growth.
作者信息
Wada A, Watanabe H
机构信息
Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Tokyo 162, Japan.
出版信息
J Bacteriol. 1998 May;180(10):2759-65. doi: 10.1128/JB.180.10.2759-2765.1998.
Abstract
pbpA, a gene encoding penicillin-binding protein (PBP) 1 of Staphylococcus aureus, was cloned in an Escherichia coli MC1061 transformant which grew on a plate containing 512 microg of vancomycin per ml. This gene encodes a 744-amino-acid sequence which conserves three motifs of PBPs, SXXK, SXN, and KTG. The chromosomal copy of pbpA could be disrupted only when RN4220, a methicillin-sensitive S. aureus strain, had additional copies of pbpA in its episome. Furthermore, these episomal copies of pbpA could not be eliminated by an incompatible plasmid when the chromosomal copy of pbpA was disrupted beforehand. Based on these observations, we concluded that pbpA is essential for the growth of methicillin-sensitive S. aureus.
摘要
pbpA基因编码金黄色葡萄球菌的青霉素结合蛋白（PBP）1，该基因在一株大肠杆菌MC1061转化子中克隆，该转化子能在每毫升含512微克万古霉素的平板上生长。该基因编码一个744个氨基酸的序列，该序列保留了PBPs的三个基序，即SXXK、SXN和KTG。只有当对甲氧西林敏感的金黄色葡萄球菌菌株RN4220在其附加体中有pbpA的额外拷贝时，pbpA的染色体拷贝才会被破坏。此外，当pbpA的染色体拷贝预先被破坏时，这些pbpA的附加体拷贝不能被不相容质粒消除。基于这些观察结果，我们得出结论，pbpA对甲氧西林敏感的金黄色葡萄球菌的生长至关重要。
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