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使用内毒素检测和精子存活试验对人类体外受精/卵胞浆内单精子注射程序进行质量控制。

Quality control of human IVF/ICSI program using endotoxin measurement and sperm survival test.

作者信息

Morimoto Y, Hayashi E, Ohno T, Kawata A, Horikoshi Y, Kanzaki H

机构信息

Centre for Reproductive Medicine and Infertility IVF Osaka Clinic, Japan.

出版信息

Hum Cell. 1997 Dec;10(4):271-6.

PMID:9573487
Abstract

Quality control is essential for maintenance and improvement of a successful human in vitro fertilization and embryo transfer (IVF/ET)/intracytoplasmic sperm injection(ICSI) program. Quality control of medium, serum and equipments were performed using endotoxin measurement and human sperm survival test. It was proved by high pregnancy rate that the method of quality control at present study was essential for the development of the program.

摘要

质量控制对于维持和改进成功的人类体外受精与胚胎移植(IVF/ET)/卵胞浆内单精子注射(ICSI)项目至关重要。通过内毒素检测和人类精子存活试验对培养基、血清和设备进行质量控制。目前研究中的质量控制方法经高妊娠率证明对该项目的发展至关重要。

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Quality control of human IVF/ICSI program using endotoxin measurement and sperm survival test.使用内毒素检测和精子存活试验对人类体外受精/卵胞浆内单精子注射程序进行质量控制。
Hum Cell. 1997 Dec;10(4):271-6.
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[A quality control for the culture system using endotoxin assays in human in vitro fertilization and embryo transfer].[在人类体外受精和胚胎移植中使用内毒素检测对培养系统进行质量控制]
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Conventional in vitro fertilization versus intracytoplasmic sperm injection in patients with borderline semen: a randomized study using sibling oocytes.精液质量临界患者常规体外受精与卵胞浆内单精子注射的比较:一项使用姐妹卵母细胞的随机研究。
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Intracytoplasmic sperm injection as a treatment for unexplained total fertilization failure or low fertilization after conventional in vitro fertilization.卵胞浆内单精子注射作为常规体外受精后不明原因的完全受精失败或低受精的一种治疗方法。
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Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates.精子染色质结构分析参数与体外受精和卵胞浆内单精子注射的受精率、胚胎质量及妊娠率无关,但可能与自然流产率有关。
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"Physiologic ICSI": hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality.“生理受精法”:透明质酸(HA)有利于选择无 DNA 碎片化和正常核的精子,从而提高胚胎质量。
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Peroxides in mineral oil used for in vitro fertilization: defining limits of standard quality control assays.矿物油中的过氧化物用于体外受精:定义标准质量控制检测的极限。
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