Tanaka A
Jpn J Exp Med. 1976 Jun;46(3):181-5.
DNA polymerase activities of intact and disrupted suspensions of the mature infectious, extracellular elementary bodies of the meningopneumonitis strain of Chlamydia psittaci were studied. Intact elementary bodies failed to incorporate labeled thymidine triphosphate (TTP), but homogenates of the organisms did incorporate TTP into the acid insoluble fraction; the reaction continued at a linear rate for 60 min and the newly synthesized DNA hybridized exclusively with DNA derived from C. psittaci elementary bodies. Synthesized DNA sedimented in 2 fractions in alkaline sucrose gradients, one in the 50S region and the other in the 10S region, corresponding to molecular weights of 2 X 10(7) and 5 X 10(5) daltons, respectively.
对鹦鹉热衣原体脑膜肺炎菌株成熟感染性细胞外原体完整和破碎悬浮液的DNA聚合酶活性进行了研究。完整的原体不能掺入标记的三磷酸胸腺嘧啶核苷（TTP），但该生物体的匀浆确实将TTP掺入了酸不溶性部分；反应以线性速率持续60分钟，新合成的DNA仅与源自鹦鹉热衣原体原体的DNA杂交。合成的DNA在碱性蔗糖梯度中沉淀为2个组分，一个在50S区域，另一个在10S区域，分别对应于2×10⁷和5×10⁵道尔顿的分子量。
