Slater J L, Griswold D J, Wojtyniak L S, Reisling M J
Regional Laboratory Services, Royal Columbian Hospital, New Westminster, British Columbia, Canada.
Transfusion. 1989 Oct;29(8):686-8. doi: 10.1046/j.1537-2995.1989.29890020440.x.
All specimens received in the blood bank over a 5-month period for crossmatch or group and screen requests were tested in parallel by a polyethylene glycol-indirect antiglobulin test (PEG-IAT) and a low-ionic-strength saline (LISS)-IAT. The sera of 41 of 1471 patients had reactions, with 50 antibodies being detected. Ten antibodies reacted only on the PEG-IAT and 14 only by the LISS-IAT; the remaining 26 antibodies were detected by both methods. Of the antibodies that reacted only by the LISS-IAT, one (anti-Jka) was considered clinically significant, whereas five of the antibodies that reacted only by the PEG-IAT (1 anti-c, 2-Fya, 1-Jkb, and 1-S) were considered significant. Two antibodies of questionable clinical significance were detected only by the PEG-IAT. In 97 percent of the sera tested, no reaction was detected by either method. The PEG-IAT is an acceptable technique for routine compatibility testing.
在5个月期间血库收到的用于交叉配血或血型鉴定及筛查请求的所有标本，均采用聚乙二醇间接抗球蛋白试验（PEG-IAT）和低离子强度盐水（LISS）-IAT进行平行检测。1471例患者中有41例血清出现反应，共检测到50种抗体。10种抗体仅在PEG-IAT中出现反应，14种仅在LISS-IAT中出现反应；其余26种抗体在两种方法中均被检测到。仅在LISS-IAT中出现反应的抗体中，有一种（抗Jka）被认为具有临床意义，而仅在PEG-IAT中出现反应的抗体中有5种（1种抗c、2种抗Fya、1种抗Jkb和1种抗S）被认为具有临床意义。仅在PEG-IAT中检测到2种临床意义存疑的抗体。在97%的检测血清中，两种方法均未检测到反应。PEG-IAT是一种可接受的常规相容性检测技术。
