基于杀稻瘟菌素脱氨酶-胸苷激酶融合体的新型哺乳动物细胞正/负选择标记
New positive/negative selectable markers for mammalian cells on the basis of Blasticidin deaminase-thymidine kinase fusions.
作者信息
Karreman C
机构信息
GSF-National Research Center for Environment and Health, Institute of Clinical Molecular Biology and Tumour Genetics, Marchioninistrasse 25, 81377 Munich, Germany.
出版信息
Nucleic Acids Res. 1998 May 15;26(10):2508-10. doi: 10.1093/nar/26.10.2508.
Abstract
Two positive and negative selectable markers were created for use in mammalian cells. They are based on two genes for the resistance to Blasticidin S (BlaS) and on the thymidine kinase (Tk) gene of herpes simplex virus (HSV). The markers can be selected positively by their ability to induce BlaS resistance and negatively on the induced sensitivity towards gancyclovir (GANC). Both constructs are also expressed in Escherichia coli and transfer BlaS resistance to this organism as well, making these markers very suitable for the construction of shuttle vectors.
摘要
构建了两种用于哺乳动物细胞的正反向选择标记。它们基于对杀稻瘟菌素S（BlaS）具有抗性的两个基因以及单纯疱疹病毒（HSV）的胸苷激酶（Tk）基因。这些标记可通过诱导对BlaS的抗性进行正向选择，并通过对更昔洛韦（GANC）诱导的敏感性进行反向选择。这两种构建体在大肠杆菌中也能表达，并且也能将BlaS抗性转移到该生物体中，使得这些标记非常适合用于构建穿梭载体。
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