人类复制起点处蛋白质 - DNA 相互作用的细胞周期调控
Cell cycle modulation of protein-DNA interactions at a human replication origin.
作者信息
Abdurashidova G, Riva S, Biamonti G, Giacca M, Falaschi A
机构信息
Molecular Biology Unit, International Centre for Genomic Engineering and Biotechnology, Trieste, Italy.
出版信息
EMBO J. 1998 May 15;17(10):2961-9. doi: 10.1093/emboj/17.10.2961.
Abstract
We followed the variations of protein-DNA interactions occurring in vivo over the early firing replication origin located near the human lamin B2 gene, in IMR-90 cells synchronized in different moments of the cell cycle. In G0 phase cells no protection is present; as the cells progress in G1 phase an extended footprint covering over 100 bp appears, particularly marked at the G1/S border. As the cells enter S phase the protection shrinks to 70 bp and remains unchanged throughout this phase. In mitosis the protection totally disappears, only to reappear in its extended form as the cells move into the next G1. These variations are reminiscent of those corresponding to the formation of the pre- and post-replicative complexes described in yeast and Xenopus cells.
摘要
我们追踪了在细胞周期不同时刻同步化的IMR-90细胞中，位于人lamin B2基因附近的早期激活复制起点处发生的体内蛋白质-DNA相互作用的变化。在G0期细胞中不存在保护作用；随着细胞进入G1期，出现了一个覆盖超过100 bp的扩展足迹，在G1/S边界处尤为明显。当细胞进入S期时，保护区域缩小到70 bp，并在整个S期保持不变。在有丝分裂过程中，保护作用完全消失，直到细胞进入下一个G1期时才以扩展形式重新出现。这些变化让人联想到酵母和非洲爪蟾细胞中描述的复制前和复制后复合物形成时的相应变化。
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本文引用的文献
1
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Cell. 1997 Oct 3;91(1):59-69. doi: 10.1016/s0092-8674(01)80009-x.
2
Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.Mcm蛋白加载到DNA复制起点受Cdc6p和周期蛋白依赖性激酶（CDKs）调控。
Cell. 1997 Aug 22;90(4):649-60. doi: 10.1016/s0092-8674(00)80526-7.
3
Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.芽殖酵母中Cdc6p介导的Mcm蛋白加载到复制前染色质上的过程。
Proc Natl Acad Sci U S A. 1997 May 27;94(11):5611-6. doi: 10.1073/pnas.94.11.5611.
4
Chromatin proteins involved in the initiation of DNA replication.参与DNA复制起始的染色质蛋白。
Curr Opin Genet Dev. 1997 Apr;7(2):152-7. doi: 10.1016/s0959-437x(97)80123-2.
5
A human protein related to yeast Cdc6p.一种与酵母Cdc6p相关的人类蛋白质。
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):142-7. doi: 10.1073/pnas.94.1.142.
6
7
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8
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9
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10
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