Roberts C, Antonoplos P
Advanced Sterilization Products, Irvine, CA 92618-9824, USA.
Am J Infect Control. 1998 Apr;26(2):94-101. doi: 10.1016/s0196-6553(98)80028-x.
Studies were conducted to determine the capability of a hydrogen peroxide gas plasma sterilization process to inactivate several types of viruses. Six test agents were used: HIV type 1, human hepatitis A virus, respiratory syncytial virus, vaccinia, herpes simplex virus type 1, and poliovirus type 2.
The test viruses were suspended in cell culture medium and dried on the bottom of sterile glass petri dishes. The inoculated dishes were processed in the hydrogen peroxide gas plasma system for half the normal sterilization cycle time. Four inoculated carriers for each virus were used in two separate half cycles. Infectivity of the test viruses and cytotoxicity to the indicator cell lines were assayed.
The hydrogen peroxide gas plasma sterilization process produced inactivation of the six viral test agents under these experimental conditions. The reduction in viral titers ranged from 2.5 log10 to 5.5 log10, a 99.68% to 99.999% decrease.
These results clearly demonstrate the virucidal effectiveness of the hydrogen peroxide gas plasma sterilization process against both lipid and nonlipid viruses.
开展了多项研究以确定过氧化氢气体等离子体灭菌工艺对多种病毒的灭活能力。使用了六种测试病原体：1型人类免疫缺陷病毒、甲型肝炎病毒、呼吸道合胞病毒、痘苗病毒、1型单纯疱疹病毒和2型脊髓灰质炎病毒。
将测试病毒悬浮于细胞培养基中，并干燥在无菌玻璃培养皿底部。接种后的培养皿在过氧化氢气体等离子体系统中处理正常灭菌周期时间的一半。每种病毒使用四个接种载体，分两个独立的半周期进行处理。检测测试病毒的感染性以及对指示细胞系的细胞毒性。
在这些实验条件下，过氧化氢气体等离子体灭菌工艺使六种病毒测试病原体失活。病毒滴度降低范围为2.5 log10至5.5 log10，即下降99.68%至99.999%。
这些结果清楚地证明了过氧化氢气体等离子体灭菌工艺对脂质和非脂质病毒均具有杀病毒效力。
