Sidwell R W, Dixon G J, Westbrook L, Dulmadge E A
Appl Microbiol. 1969 Jun;17(6):790-6. doi: 10.1128/am.17.6.790-796.1969.
A quantitative, reproducible method was developed for the evaluation of the virucidal activity of test gases. Using this method, we determined the virucidal effectiveness of a Steri-Vac ethylene oxide gas sterilizer. Wool gabardine material was exposed to high concentrations of herpes simplex, vaccinia, parainfluenza, or polio viruses and was processed through the sterilizer. Two time-temperature cycles of the machine, 29 C for 180 min and 60 C for 48 min, were used in separate experiments. The viruses were exposed to the gas when freshly pipetted onto the fabric or when pipetted on the material and allowed to dry 16 to 24 hr. In two experiments carried out under each condition, the virus titers were reduced by the sterilization process to less than detectable limits. These titer reductions were for the herpes virus >/= 2.7 to 5.0 log, for vaccinia virus >/= 4.0 to 6.1 log, for parainfluenza virus >/= 1.8 to 4.9 log, and for poliovirus >/= 4.9 to 7.7 log. The observed reductions in virus titers were the same whether the virus-contaminated fabrics were sealed in polyethylene packages or held in open petri dishes during exposure to ethylene oxide.
开发了一种定量、可重复的方法来评估测试气体的杀病毒活性。使用该方法，我们测定了Steri-Vac环氧乙烷气体灭菌器的杀病毒效果。将羊毛华达呢材料暴露于高浓度的单纯疱疹病毒、痘苗病毒、副流感病毒或脊髓灰质炎病毒中，然后通过灭菌器进行处理。在单独的实验中使用了该机器的两个时间-温度循环，即29℃180分钟和60℃48分钟。将病毒新鲜移液到织物上或移液到材料上并使其干燥16至24小时后，使其暴露于气体中。在每种条件下进行的两项实验中，灭菌过程使病毒滴度降低到低于可检测极限。这些滴度降低对于疱疹病毒≥2.7至5.0对数，痘苗病毒≥4.0至6.1对数，副流感病毒≥1.8至4.9对数，脊髓灰质炎病毒≥4.9至7.7对数。无论受病毒污染的织物在暴露于环氧乙烷期间是密封在聚乙烯包装中还是放在开放的培养皿中，观察到的病毒滴度降低都是相同的。
