Shalhav M, Fuss Z, Weiss E I
Section of Oral Biology, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
J Endod. 1997 Oct;23(10):616-9. doi: 10.1016/S0099-2399(97)80172-0.
The aim of this study was to evaluate the antibacterial activity of a recently introduced glass ionomer endodontic sealer, Ketac Endo (KE), compared to a commonly used ZOE-based endodontic sealer, Roth's cement (RC). With the use of E. faecalis as a test organism, the agar diffusion test (ADT) and direct contact test (DCT) were performed. For the DCT, the sealers were mixed and placed on the side wall of microtiter plate wells. A 10 microL bacterial suspension was placed on the tested material samples 20 min, 24 h, and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1 h at 37 degrees C. Fresh media was then added and bacterial growth was measured every 30 min over 15 h. The results showed that in the ADT, freshly mixed KE exhibited a twofold greater inhibition zone than RC (p < 0.05); in the DCT, freshly mixed KE and RC completely inhibited bacterial growth. The 24-h and 7-day samples of KE showed no antibacterial activity, whereas RC continued to exhibit a strong effect with similar samples. It has been concluded that KE possesses a short-acting very potent and diffusable antibacterial activity, whereas RC extends its effect over 7 days after setting.
本研究的目的是评估一种最近推出的玻璃离子根管封闭剂Ketac Endo（KE）与常用的基于氧化锌丁香酚的根管封闭剂Roth's cement（RC）相比的抗菌活性。以粪肠球菌作为测试微生物，进行了琼脂扩散试验（ADT）和直接接触试验（DCT）。对于DCT，将封闭剂混合后放置在微量滴定板孔的侧壁上。在混合后20分钟、24小时和7天，将10微升细菌悬液放置在测试材料样品上。使细菌在37℃下与封闭剂直接接触1小时。然后加入新鲜培养基，并在15小时内每30分钟测量一次细菌生长情况。结果表明，在ADT中，新鲜混合的KE表现出比RC大两倍的抑菌圈（p < 0.05）；在DCT中，新鲜混合的KE和RC完全抑制了细菌生长。KE的24小时和7天样品没有抗菌活性，而RC在类似样品中继续表现出很强的效果。得出的结论是，KE具有短效的非常强效且可扩散的抗菌活性，而RC在凝固后7天内仍能发挥作用。
