Pizzo Giuseppe, Giammanco Giovanni M, Cumbo Enzo, Nicolosi Giuseppe, Gallina Giuseppe
Department of Oral Sciences, University of Palermo, Via del Vespro 129, 90127 Palermo, Italy.
J Dent. 2006 Jan;34(1):35-40. doi: 10.1016/j.jdent.2005.03.001.
To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomèthasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare).
A direct contact test (DCT) was performed. A 10 microl suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1h at 37 degrees C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well.
All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing.
The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.
评估四种根管封闭剂的抗菌活性：一种环氧树脂封闭剂（AH Plus）、两种氧化锌丁香酚（ZOE）基封闭剂（Endomèthasone、牙髓根管封闭剂）和一种同时含有ZOE和邻苯基苯酚的封闭剂（Vcanalare）。
进行直接接触试验（DCT）。在混合后20分钟、24小时和7天时，将10微升粪肠球菌悬液置于测试材料上。使细菌在37摄氏度下与封闭剂直接接触1小时。然后每隔30分钟用分光光度法测量细菌生长7小时，并在24小时后再次测量。
所有新混合的封闭剂均显示出对细菌生长的完全抑制。24小时龄的样品也得到了类似的结果，但AH Plus除外。Vcanalare是混合7天后仍能抑制细菌生长的唯一封闭剂。
测试封闭剂的抗菌活性取决于混合和测试之间的时间间隔。所有封闭剂在新混合时均表现出杀菌作用，但只有Vcanalare能将这种作用延长至凝固后7天。
