Department of Endodontics, School of Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Int Endod J. 2011 Dec;44(12):1128-33. doi: 10.1111/j.1365-2591.2011.01931.x. Epub 2011 Sep 5.
To evaluate the pH and antibacterial activity of Endo CPM Sealer and MTA Fillapex by two different methods, using white MTA and Endofill as references for comparison.
Antibacterial activity was evaluated against Enterococcus faecalis (ATCC 29212). The agar diffusion test (ADT) was performed to evaluate the effect before setting. The materials were placed in four equidistant wells made in ten agar plates. After incubation at 37 °C for 48 h, the inhibition zones were measured using a digital paquimeter. The direct contact test (DCT) was performed to assess the antibacterial effect after setting. Suspensions of crushed materials were prepared and mixed with E. faecalis. After different periods of time (1, 6, 15 and 60 min), the survival of bacteria was assessed by using 10-fold serial dilution and cultivated on agar plates in triplicate. Colony-forming units (CFU) mL(-1) were calculated after incubation. pH values were also measured in triplicate. Comparison between sealers in the ADT and DCT was performed by the Kruskal-Wallis test.
In the ADT, inhibition zones were found with MTA Fillapex and Endofill. They were similar to each other and greater than the other sealers (P < 0.05). None of the tested sealers demonstrated antibacterial activity in the DCT, and thus, all sealers had similar bacterial counts compared with the negative control group (P > 0.05). White MTA and Endo CPM Sealer suspensions had pH values >11, whilst MTA Fillapex and Endofill had lower values.
MTA Fillapex and Endofill had an antibacterial effect against E. faecalis before setting, but none of the sealers maintained antibacterial activity after setting, despite the high pH of the MTA-based materials.
通过两种不同方法评估 Endo CPM 根管封闭剂和 MTA Fillapex 的 pH 值和抗菌活性，以白色 MTA 和 Endofill 为参照进行比较。
采用琼脂扩散试验（ADT）评估未凝固状态下的抗菌活性，以 Enterococcus faecalis（ATCC 29212）为受试菌。将材料分别放置于十个琼脂平板中，每平板等距的四个孔内。孵育 37°C 48 h 后，用数字 paquimeter 测量抑菌圈直径。直接接触试验（DCT）评估凝固后抗菌效果，将粉碎后的材料悬液与 E. faecalis 混合，于 1、6、15 和 60 min 时分别取样，10 倍稀释后接种于琼脂平板，3 个重复，孵育后计算活菌数（CFU）。采用三重复 pH 值测量。ADT 中各封剂间比较采用 Kruskal-Wallis 检验，DCT 中采用方差分析。
ADT 中，MTA Fillapex 和 Endofill 出现抑菌圈，二者相似，大于其余封剂（P < 0.05）。DCT 中，所有封剂均无抗菌活性，与阴性对照组相比，所有封剂的活菌数均无显著差异（P > 0.05）。白色 MTA 和 Endo CPM 根管封闭剂悬液 pH 值>11，而 MTA Fillapex 和 Endofill 的 pH 值较低。
凝固前，MTA Fillapex 和 Endofill 对 E. faecalis 具有抗菌活性，但凝固后所有封剂均无抗菌活性，尽管 MTA 基材料 pH 值较高。
