Oki A, Nishida M, Satoh T, Tsunoda H, Kasahara K, Saijo K, Kubo T, Ohno T
RIKEN Cell Bank, The Institute of Physical and Chemical Research, Tsukuba Science City, Ibaraki, Japan.
In Vitro Cell Dev Biol Anim. 1998 Apr;34(4):290-7. doi: 10.1007/s11626-998-0005-1.
A novel human cell line, TOM-2, was established from a rare uterine cervical cancer, glassy cell carcinoma (GCC). TOM-2 is the second established GCC cell line so far reported. The cells were intermediately or poorly differentiated with dysplastic nuclei and polygonal shape and secreted two tumor markers and cytokines, i.e., CA-125 and SCC, interleukin (1L)-1alpha, -6, and -8, and TNF-alpha. Growth of TOM-2 was so strongly dependent on population density that it was not possible to determine the plating efficiency. In mass culture, the following characteristics were observed: doubling time, 83 h; mode of chromosome number, 79; human papillomavirus type 18 DNA, detectable; tumorigenicity, easily transplantable into subcutis of nude mice; chemosensitivity in vitro, considerably sensitive to Cisplatin and 5-FU but not to 9 other antineoplastic agents. This novel cell line will be useful for developing new therapeutic strategies for the rare cancer, GCC.
一种新的人类细胞系TOM-2，是从一种罕见的子宫颈癌——玻璃样细胞癌（GCC）中建立的。TOM-2是迄今为止报道的第二个已建立的GCC细胞系。这些细胞中度或低分化，核发育异常，呈多边形，分泌两种肿瘤标志物和细胞因子，即CA-125和鳞状细胞癌抗原（SCC）、白细胞介素（IL）-1α、-6和-8以及肿瘤坏死因子-α（TNF-α）。TOM-2的生长强烈依赖于群体密度，以至于无法确定接种效率。在大规模培养中，观察到以下特征：倍增时间为83小时；染色体数目模式为79；可检测到人乳头瘤病毒18型DNA；具有致瘤性，易于移植到裸鼠皮下；体外化学敏感性，对顺铂和5-氟尿嘧啶相当敏感，但对其他9种抗肿瘤药物不敏感。这种新的细胞系将有助于为罕见癌症GCC开发新的治疗策略。
