Starkey M P, Umrania Y, Mundy C R, Bishop M J
UK HGMP Resource Centre, Hinxton, Cambridge, UK.
Mol Biotechnol. 1998 Feb;9(1):35-57. doi: 10.1007/BF02752696.
cDNA libraries are the cornerstone of efforts to identify the relatively small regions of genomes that are responsible for biological effects. Gene hunter seeking candidate genes, via a variety of approaches, ultimately focus on the cloning, sequencing, and expression of cDNAs. Assistance is now available to researchers in the form of genome programs, whose initial goals include assembly of a complete collection of expressed sequences derived from the genome of interest. The concept of reference sets of cDNA libraries is that the aims of genome programs are served most effectively by different laboratories working on a common set of high-quality arrayed cDNA libraries, using different experimental approaches, thereby reducing unnecessary duplication of effort, and maximizing the amount of information that one set of resources can provide.
互补DNA（cDNA）文库是识别基因组中负责生物学效应的相对较小区域的工作基石。基因猎手通过各种方法寻找候选基因，最终聚焦于cDNA的克隆、测序和表达。现在，基因组计划以一种形式为研究人员提供了帮助，其最初目标包括组装来自感兴趣基因组的完整表达序列集合。cDNA文库参考集的概念是，不同实验室使用不同实验方法研究一组共同的高质量阵列cDNA文库，能最有效地实现基因组计划的目标，从而减少不必要的重复工作，并最大限度地提高一组资源所能提供的信息量。
