Bao L, Zhang Y, Zheng X
Renal Division, First Teaching Hospital, Beijing Medical University.
Zhonghua Nei Ke Za Zhi. 1996 Aug;35(8):542-4.
In order to demonstrate whether Epstein-Barr virus (EBV) infection might play a role in the pathogenesis of interstitial nephritis as suggested by many scholars, EBV DNA was detected in twelve specimens of frozen renal biopsy tissue from patients with interstitial nephritis by using nest polymerase chain reaction (nest PCR). For comparison, frozen renal biopsy tissue from ten patients with minimal change disease was used as control. Southern blot hybridization was used to check the specificity of PCR product. The results showed that eight of twelve frozen renal biopsy specimens from interstitial nephritis patients were EBV DNA positive (66.7%), as compared with negative in all the ten frozen renal specimens from minimal change disease patients. The differences was statistically significant (P < 0.01). The study strongly suggests that EBV infection may play an important role in the pathogenesis of interstitial nephritis. The location of EBV in renal tissue and the mechanism inducing interstitial nephritis by EBV are to be clarified.
为了证实许多学者所提出的EB病毒（EBV）感染是否可能在间质性肾炎的发病机制中起作用，采用巢式聚合酶链反应（巢式PCR）对12例间质性肾炎患者的冷冻肾活检组织标本进行EBV DNA检测。作为对照，采用10例微小病变病患者的冷冻肾活检组织。用Southern印迹杂交法检测PCR产物的特异性。结果显示，12例间质性肾炎患者的冷冻肾活检标本中有8例EBV DNA阳性（66.7%），而10例微小病变病患者的冷冻肾标本均为阴性。差异有统计学意义（P<0.01）。该研究强烈提示EBV感染可能在间质性肾炎的发病机制中起重要作用。EBV在肾组织中的定位以及EBV诱发间质性肾炎的机制有待阐明。
