de Bethune M P, Nierhaus K H
Eur J Biochem. 1978 May;86(1):187-91. doi: 10.1111/j.1432-1033.1978.tb12298.x.
Virginiamycin S is an inhibitor of protein synthesis in vivo. In this paper we show by equilibrium dialysis that it binds specifically to the 50-S subunit of Escherichia coli ribosomes, with one binding site per subunit. This binding is not altered by the presence of chloramphenicol, tetracycline or puromycin but is competed for by erythromycin. Using the splitting-reconstitution method, it could be demonstrated that protein L16 is absolutely required for the binding of virginiamycin S to the 50-S subunit.
维吉尼亚霉素S是体内蛋白质合成的抑制剂。在本文中，我们通过平衡透析表明，它特异性地结合到大肠杆菌核糖体的50-S亚基上，每个亚基有一个结合位点。氯霉素、四环素或嘌呤霉素的存在不会改变这种结合，但红霉素会与之竞争。使用拆分-重组方法，可以证明蛋白质L16是维吉尼亚霉素S与50-S亚基结合所绝对必需的。
