通过碳-13核磁共振光谱法测定新型隐球菌葡糖醛酸木甘露聚糖合成中木糖、甘露糖和甘露醇的同化作用。
Assimilation of xylose, mannose, and mannitol for synthesis of glucuronoxylomannan of Cryptococcus neoformans determined by 13C nuclear magnetic resonance spectroscopy.
作者信息
Cherniak R, O'Neill E B, Sheng S
机构信息
Laboratory for Biological and Chemical Sciences, Department of Chemistry, Georgia State University, Atlanta, Georgia 30303, USA.
出版信息
Infect Immun. 1998 Jun;66(6):2996-8. doi: 10.1128/IAI.66.6.2996-2998.1998.
Abstract
Cryptococcus neoformans NIH 409 was cultured in a defined medium containing D-[1-13C]xylose (Xyl), D-[1-13C]mannose (Man), or D-[1-13C]mannitol as the sole carbon source. The distribution of 13C in the Man, Xyl, glucuronic acid (GlcA), and O-acetyl constituents of native and de-O-acetylated glucuronoxylomannan (GXM) was determined by one-dimensional 13C nuclear magnetic resonance spectroscopy. The carbon chain of Man was incorporated intact into GXM since 13C was observed only in carbon 1 of Man, GlcA, and Xyl. The carbon chain of mannitol was incorporated intact into GXM since 13C was observed only in carbons 1 and 6. This was expected since mannitol has an axis of symmetry. The carbon chain of Xyl was identified only in carbons 1 and 3 of Man, GlcA, and Xyl. This pattern of labeling is consistent with the assimilation of Xyl through the pentophosphate pathway.
摘要
新型隐球菌NIH 409在含有D-[1-¹³C]木糖（Xyl）、D-[1-¹³C]甘露糖（Man）或D-[1-¹³C]甘露醇作为唯一碳源的限定培养基中培养。通过一维¹³C核磁共振光谱法测定了天然和脱O-乙酰化葡糖醛酸木聚糖甘露聚糖（GXM）中¹³C在Man、Xyl、葡糖醛酸（GlcA）和O-乙酰成分中的分布。Man的碳链完整地并入GXM中，因为仅在Man、GlcA和Xyl的碳1中观察到¹³C。甘露醇的碳链完整地并入GXM中，因为仅在碳1和6中观察到¹³C。由于甘露醇具有对称轴，所以这是预期的结果。Xyl的碳链仅在Man、GlcA和Xyl的碳1和3中被鉴定出来。这种标记模式与通过磷酸戊糖途径同化Xyl是一致的。
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