Souhrada J F, Dickey D W
Respir Physiol. 1976 Aug;27(2):241-51. doi: 10.1016/0034-5687(76)90077-3.
Isolated tracheal preparations (ITP) were obtained from control guinea pigs and animals with acute and chronic experimental asthma (AA and CHA). It was observed that after in vitro antigen challenge, in addition to the classic Schultz-Dale response (the immediate increase in the tone of sensitized airway smooth muscle), a significant increase of the active state of airway smooth muscle was seen. This active state was observed as the potentiated, phasic, mechanical activities of ITP and was evaluated by both maximum active tension (AT max) and maximum rate of tension development (dT/dt). No apparent functional differences in the tonic response or the active state of airway smooth muscle between AA and CHA animals were found. Tracheas isolated from animals with CHA demonstrated smooth muscle hypertrophy as measured by wet, dry and relative weights of the tracheas. The repeated administration of antigen into the experimental bath was ineffective in inducing any physiological changes. The administration of histamine to ITP from control animals induced changes of mechanical activities of trachea comparable to those seen in both AA and CHA animals.
从对照豚鼠以及患有急性和慢性实验性哮喘（AA和CHA）的动物身上获取离体气管制备物（ITP）。观察到，在体外抗原激发后，除了经典的舒尔茨-戴尔反应（致敏气道平滑肌张力立即增加）外，还可见气道平滑肌活性状态显著增加。这种活性状态表现为ITP的增强、阶段性机械活动，并通过最大主动张力（AT max）和最大张力发展速率（dT/dt）进行评估。未发现AA和CHA动物在气道平滑肌的紧张性反应或活性状态方面存在明显功能差异。通过气管的湿重、干重和相对重量测量发现，从CHA动物分离的气管表现出平滑肌肥大。在实验浴中反复给予抗原未能诱导任何生理变化。向对照动物的ITP中给予组胺可诱导气管机械活动发生变化，与AA和CHA动物中观察到的变化相当。
