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将DNA插入痘苗病毒。

The insertion of DNA into vaccinia virus.

作者信息

Morgan C

出版信息

Science. 1976 Aug 13;193(4253):591-2. doi: 10.1126/science.959819.

DOI:10.1126/science.959819
PMID:959819
Abstract

Cells infected with vaccinia virus in the presence of hydroxyurea (HU), which blocks DNA replication, were examined in thin sections by electron microscopy at intervals after removal of HU. Dense, fibrillar material was observed at the orifice formed just before closure of the membrane constituting the envelope of the immature form of the virus. It is concluded that synchrony of assembly enabled stages in the condensation and insertion of viral deoxyribonucleoprotein to be observed. The mechanism appears to be similar to that encountered in morphologic studies of herpes simplex virus and in biochemical studies of poliovirus, adenovirus, and several bacteriophages.

摘要

在存在羟基脲（HU）的情况下感染痘苗病毒的细胞，羟基脲会阻断DNA复制，在去除HU后的不同时间间隔通过电子显微镜对薄切片进行检查。在构成未成熟病毒包膜的膜即将封闭之前形成的孔口处观察到致密的纤维状物质。得出的结论是，装配同步性使得能够观察到病毒脱氧核糖核蛋白凝聚和插入的各个阶段。其机制似乎与在单纯疱疹病毒的形态学研究以及脊髓灰质炎病毒、腺病毒和几种噬菌体的生化研究中所遇到的机制相似。

相似文献

1
The insertion of DNA into vaccinia virus.将DNA插入痘苗病毒。
Science. 1976 Aug 13;193(4253):591-2. doi: 10.1126/science.959819.
2
Biochemical and electron microscopic observations of vaccinia virus morphogenesis in HeLa cells after hydroxyurea reversal.羟基脲逆转后HeLa细胞中牛痘病毒形态发生的生化与电子显微镜观察
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Biogenesis of vaccinia: relationship of the envelope to virus assembly.痘苗病毒的生物发生：包膜与病毒装配的关系。
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Vaccinia virus reexamined: development and release.痘苗病毒再审视：发育与释放
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Electron microscopic studies on assembly of herpes simplex virus upon removal of hydroxyurea block.去除羟基脲阻断后单纯疱疹病毒装配的电子显微镜研究
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The block in assembly of modified vaccinia virus Ankara in HeLa cells reveals new insights into vaccinia virus morphogenesis.安卡拉改良痘苗病毒在HeLa细胞中的组装受阻揭示了痘苗病毒形态发生的新见解。
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Synergic action of distamycin A and hydroxyurea on the reproduction of DNA viruses in cell cultures.放线菌素A与羟基脲对细胞培养中DNA病毒复制的协同作用。
Acta Virol. 1975 Jan;19(1):73-7.
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The inhibition of vaccinia virus replication by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB): an effect at the assembly stage.5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DRB）对痘苗病毒复制的抑制作用：在装配阶段的效应
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Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides.牛痘病毒多肽合成：复制前和复制后多肽的顺序出现及稳定性
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Vaccinia virus mutants with alanine substitutions in the conserved G5R gene fail to initiate morphogenesis at the nonpermissive temperature.在保守的G5R基因中具有丙氨酸替代的痘苗病毒突变体在非允许温度下无法启动形态发生。
J Virol. 2004 Oct;78(19):10238-48. doi: 10.1128/JVI.78.19.10238-10248.2004.

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Poxvirus DNA replication.痘病毒 DNA 复制。
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Analysis of viral membranes formed in cells infected by a vaccinia virus L2-deletion mutant suggests their origin from the endoplasmic reticulum.分析感染牛痘病毒 L2 缺失突变体的细胞中形成的病毒膜，提示它们可能来自内质网。
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J Virol. 2011 Mar;85(6):2504-11. doi: 10.1128/JVI.02505-10. Epub 2011 Jan 12.
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