Márquez F J, Muniain M A, Soriguer R C, Izquierdo G, Rodríguez-Baño J, Borobio M V
Estación Biológica Doñana, Consejo Superior de Investigaciones Científicas, Sevilla, Spain.
Am J Trop Med Hyg. 1998 May;58(5):570-7. doi: 10.4269/ajtmh.1998.58.570.
An undescribed rickettsia was directly analyzed with specific rickettsial molecular biology tools on Ixodes ricinus L. collected in different localities of the province of Cadiz (southwestern Spain). On the basis of the results of the citrate synthase (glta) gene, 190 kD-outer membrane protein (rOmpA) gene, and 16S ribosomal RNA (16S rRNA) gene partial sequence data, it was found that this rickettsia is sufficiently genetically distinct from other Rickettsia to be considered a distinct taxonomic entity. The isolation and culture of this organism, as well as comparative antigenic analysis, are required to ensure its conclusive taxonomic placement among spotted fever rickettsiae. The epidemiologic role of this new rickettsial agent and its possible pathogenicity to wild and domestic animals or humans is still unknown and needs to be investigated.
利用特定的立克次氏体分子生物学工具，对采自西班牙西南部加的斯省不同地点的蓖麻硬蜱（Ixodes ricinus L.）体内一种未描述过的立克次氏体进行了直接分析。根据柠檬酸合酶（glta）基因、190 kD外膜蛋白（rOmpA）基因和16S核糖体RNA（16S rRNA）基因部分序列数据的结果，发现这种立克次氏体在遗传上与其他立克次氏体有足够的差异，可被视为一个独特的分类实体。需要对该生物体进行分离培养以及进行比较抗原分析，以确保其在斑点热立克次氏体中的最终分类定位。这种新型立克次氏体病原体的流行病学作用及其对野生动物、家畜或人类可能的致病性仍然未知，有待研究。
