Ensell M X, Whong W Z, Heng Z C, Nath J, Ong T
West Virginia University, Morgantown, USA.
Mutat Res. 1998 Feb 13;412(3):283-91. doi: 10.1016/s1383-5718(97)00200-3.
The rat tracheal epithelial (RTE) cell transformation assay was performed to determine the transforming activity of diesel emission particles (DEPs) and two related compounds, 1-nitropyrene (1-NP) and dibenzo(a,i)pyrene (DBP). RTE cells were treated with these agents in vitro and in vivo. Transformed cells from foci induced by these agents were passaged over 20 times to establish immortal cell lines. Results show that (1) DEPs- and 1-NP-induced cell transformation only with the in vivo exposure (30-75 mg/kg bw DEPs and 15-60 mg/kg bw 1-NP); (2) positive dose-related responses to DBP were found with both in vitro (0.05-0.50 microg/ml) and in vivo (7.5-30 mg/kg bw) exposures; (3) the fraction of transformed foci becoming cell lines was in the order of 1-NP(25/48) > DBP(8/28) > DEPs(0/30). These results indicate that (1) DEPs, 1-NP and DBP are capable of transforming rat tracheal epithelial cells, however, the transforming activity of DEPs and -NP may be dependent on metabolic activation, and (2) transformed cells induced by DEPs have a very low probability, if any, of becoming cell lines.
进行大鼠气管上皮（RTE）细胞转化试验，以确定柴油排放颗粒（DEP）以及两种相关化合物1-硝基芘（1-NP）和二苯并（a，i）芘（DBP）的转化活性。RTE细胞在体外和体内用这些试剂进行处理。由这些试剂诱导形成的病灶中的转化细胞传代20次以上以建立永生化细胞系。结果表明：（1）仅在体内暴露（30 - 75 mg/kg体重的DEP和15 - 60 mg/kg体重的1-NP）时，DEP和1-NP可诱导细胞转化；（2）在体外（0.05 - 0.50 μg/ml）和体内（7.5 - 30 mg/kg体重）暴露时，均发现对DBP有阳性剂量相关反应；（3）转化病灶形成细胞系的比例顺序为1-NP（25/48）> DBP（8/28）> DEP（0/30）。这些结果表明：（1）DEP、1-NP和DBP能够转化大鼠气管上皮细胞，然而，DEP和1-NP的转化活性可能依赖于代谢激活，（2）由DEP诱导的转化细胞形成细胞系的概率非常低（如果有的话）。
