Wu C A, Shanley J D
Division of Infectious Diseases, Department of Medicine, University of Connecticut Health Center, Farmington 06030-3212, USA.
J Gen Virol. 1998 May;79 ( Pt 5):1247-56. doi: 10.1099/0022-1317-79-5-1247.
Human herpesvirus-6 (HHV-6) exhibits a predominant tropism for CD4+ T-lymphocytes, but can infect other components of the blood as well as surrounding tissue and organs. To understand the role of the endothelium in the transmission and haematogenous spread of this virus, human umbilical vein endothelial cells (HUVEC) were infected with HHV-6 and monitored for viral gene expression. The presence of both early and late viral antigens was demonstrated by indirect immunofluorescence in 37.6 and 6.5%, respectively, of HUVEC. However, attempts to detect the release of infectious virus were not successful, indicating infection is semipermissive in nature. Upon continued passage of infected HUVEC monolayers, HHV-6 antigen-positive cells persisted up to 27 days post-infection. Furthermore, the virus could be recovered from HUVEC monolayers that contained fewer than 1% antigen-positive cells by co-cultivation with peripheral blood mononuclear cells. Together, these findings suggest that endothelial cells may serve as a reservoir for harbouring HHV-6.
人类疱疹病毒6型（HHV - 6）主要感染CD4 + T淋巴细胞，但也可感染血液中的其他成分以及周围组织和器官。为了解内皮细胞在该病毒传播和血行播散中的作用，用人脐静脉内皮细胞（HUVEC）感染HHV - 6，并监测病毒基因表达。通过间接免疫荧光法分别在37.6%和6.5%的HUVEC中检测到早期和晚期病毒抗原的存在。然而，检测感染性病毒释放的尝试未成功，表明感染本质上是半允许性的。在持续传代感染的HUVEC单层细胞后，HHV - 6抗原阳性细胞在感染后持续存在长达27天。此外，通过与外周血单个核细胞共培养，可从抗原阳性细胞少于1%的HUVEC单层细胞中分离出病毒。这些发现共同表明，内皮细胞可能是HHV - 6的储存库。
