Isaac-Renton J, Bowie W R, King A, Irwin G S, Ong C S, Fung C P, Shokeir M O, Dubey J P
Department of Pathology and Laboratory Medicine, University of British Columbia, British Columbia Centre for Disease Control, British Columbia Ministry of Health, Vancouver, Canada.
Appl Environ Microbiol. 1998 Jun;64(6):2278-80. doi: 10.1128/AEM.64.6.2278-2280.1998.
The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gondii. The method developed was based on the current U.S. Environmental Protection Agency method for detection of Cryptosporidium oocysts. Collection of large-volume drinking water samples and cartridge filter processing were unchanged, although identification of Toxoplasma oocysts in the filter retentate was carried out by using a previously described rodent model. Validation of the method developed was tested by using oocysts from a well-characterized Toxoplasma strain.
全球最大规模的水源性弓形虫病疫情发生在加拿大西部不列颠哥伦比亚省的一个直辖市。在疫情调查过程中，当饮用水被确定为可能的感染源时，需要一种实验室方法来尝试检测寄生虫刚地弓形虫。所开发的方法基于美国环境保护局现行的检测隐孢子虫卵囊的方法。大容量饮用水样本的采集和滤芯处理过程保持不变，不过通过使用先前描述的啮齿动物模型对滤芯截留物中的弓形虫卵囊进行鉴定。所开发方法的验证是通过使用来自一个特征明确的弓形虫菌株的卵囊来进行的。
