通过将流式分选染色体衍生的微卫星标记整合到国际牛基因图谱中获得的牛1号染色体的细胞遗传学定位遗传图谱。

A cytogenetically anchored genetic map of bovine chromosome 1 obtained by integrating flow-sorted chromosome-derived microsatellite markers into the international bovine map.

作者信息

Vaiman D, Schibler L, Oustry A, Schmitz A, Furet J P, Barendse W, Cribiu E P

机构信息

Département de Génétique Animale, INRA-CRJ, Jouy-en-Josas, France.

出版信息

Cytogenet Cell Genet. 1997;79(3-4):204-7. doi: 10.1159/000134722.

DOI:10.1159/000134722

PMID:9605852

Abstract

A genomic library was constructed from a peak of flow-sorted bovine chromosomes 1 + X after PCR amplification. Forty-three bovine chromosome 1 microsatellites were isolated, genetically mapped and integrated in the international genetic map. In addition, BAC clones from a goat BAC library were identified for five markers (DVEPC119, INRA011, BM4307, KAP8 and MAF64). These goat BACs could be mapped by FISH onto bovine chromosome 1 to bands 1q44-->q45, 1q25, 1q21, 1q12 and 1q14-->q21, respectively. This map reduces the average interval between consecutive markers on the international bovine genetic map from 5.5 cM to 2.5 cM, and provides a good starting point for positional cloning projects in cattle, sheep or goats.

摘要

在PCR扩增后，从流式分选的牛1号染色体+X的峰值构建了基因组文库。分离出43个牛1号染色体微卫星，进行基因定位并整合到国际遗传图谱中。此外，从山羊BAC文库中鉴定出五个标记（DVEPC119、INRA011、BM4307、KAP8和MAF64）的BAC克隆。这些山羊BAC可通过荧光原位杂交分别定位到牛1号染色体的1q44→q45、1q25、1q21、1q12和1q14→q21带。该图谱将国际牛遗传图谱上连续标记之间的平均间隔从5.5厘摩减少到2.5厘摩，并为牛、绵羊或山羊的定位克隆项目提供了一个良好的起点。