Saito T, Bjarkam C R, Nakamura M, Nemoto T
Laboratory of Neurophysiology, National Institute of Animal Industry, Ibaraki, Japan.
J Neurosci Methods. 1998 Mar 13;80(1):29-36. doi: 10.1016/s0165-0270(97)00183-0.
In this study a stereotaxic instrument and a stereotaxic procedure based on external skull structures, to be used in prepubertal male Landrace pigs weighing less than 30 kg, is described. The instrument represents an adaptation of the apparatus designed by Marcilloux et al., Brain Res Bull 1989;22:591-597, but we have modified the instrument for stereotaxic procedures based on external skull structures, instead of intracerebral structures necessitating ventriculography (Marcilloux et al., Brain Res Bull 1989;22:591-597). For this reason the U-shaped frame and the ear-bar supports have been changed allowing the three-dimensional placement of the ear-bars into the oblique auditory canals. Firm fixation of the skulls of pigs weighing less than 30 kg, was furthermore secured with modified infraorbital ridges and hard palate pieces. Measurements of distances between external skull structures in animals of the same sex, age and weight showed a negligible variation, thus enabling definition of the horizontal, frontal and sagittal zero planes using external skull structures alone. Stereotaxic coordinates for the hippocampal region of male Landrace pigs weighing 10 kg were then provided and the coordinates from two different levels of the hippocampal region are presented in the text. The reliability of the stereotaxic instrument was finally secured by intrahippocampal injections of ink at predetermined coordinates.
本研究描述了一种立体定位仪及基于颅骨外部结构的立体定位方法，用于体重小于30kg的青春期前雄性长白猪。该仪器是对Marcilloux等人（《脑研究通报》，1989年；22:591 - 597）所设计仪器的改进，但我们对其进行了修改，使其适用于基于颅骨外部结构而非需要脑室造影的脑内结构的立体定位操作（Marcilloux等人，《脑研究通报》，1989年；22:591 - 597）。因此，U形框架和耳杆支撑结构已被改变，使耳杆能够三维放置到斜向耳道中。此外，通过改良眶下嵴和硬腭片确保了体重小于30kg的猪颅骨的牢固固定。对相同性别、年龄和体重的动物颅骨外部结构之间的距离测量显示差异可忽略不计，从而仅使用颅骨外部结构就能确定水平、额部和矢状零平面。随后给出了体重10kg雄性长白猪海马区的立体定位坐标，并在文中呈现了海马区两个不同层面的坐标。最后通过在预定坐标处向海马内注射墨水确保了立体定位仪的可靠性。
