Townsend D E, Naqui A
IDEXX Laboratories, Inc., Westbrook, ME 04092-2041, USA.
J AOAC Int. 1998 May-Jun;81(3):563-9.
The SimPlate Total Plate Count (TPC) test, developed by IDEXX Laboratories, Inc., detects and quantitates total bacterial concentration in food after 24 h of incubation. The performance of SimPlate TPC was compared with that of the plate count agar (PCA) method for enumerating total bacterial concentration of 255 food samples representing 15 different food matrixes. Total bacterial counts on SimPlate TPC were measured after 24 h of incubation and plotted against values obtained from PCA after 48 h. Simple regression analysis of the data showed strong correlation between the methods (r = 0.95); the sensitivity of SimPlate TPC for foodborne bacteria was 96% relative to PCA (slope = 0.96). It was concluded that SimPlate TPC is a suitable alternative for the detection and quantitation of foodborne bacteria. The method has been granted Performance Tested Certification by the AOAC Research Institute.
由IDEXX实验室公司开发的SimPlate总平板计数（TPC）测试，可在培养24小时后检测并定量食品中的总细菌浓度。将SimPlate TPC的性能与平板计数琼脂（PCA）方法进行了比较，以测定代表15种不同食品基质的255个食品样本中的总细菌浓度。在培养24小时后测量SimPlate TPC上的总细菌数，并与48小时后从PCA获得的值进行对比绘图。对数据进行的简单回归分析表明，两种方法之间具有很强的相关性（r = 0.95）；相对于PCA，SimPlate TPC对食源细菌的灵敏度为96%（斜率 = 0.96）。得出的结论是，SimPlate TPC是检测和定量食源细菌的合适替代方法。该方法已获得AOAC研究所的性能测试认证。
