SimPlate 总平板计数法与Petrifilm、Redigel以及传统倾注平板法在食品中需氧微生物计数方面的比较。

Comparison of the SimPlate total plate count method with Petrifilm, Redigel, conventional pour-plate methods for enumerating aerobic microorganisms in foods.

作者信息

Beuchat L R, Copeland F, Curiale M S, Danisavich T, Gangar V, King B W, Lawlis T L, Likin R O, Okwusoa J, Smith C F, Townsend D E

机构信息

Center for Food Safety and Quality Enhancement, University of Georgia, Griffin 30223-1797, USA.

出版信息

J Food Prot. 1998 Jan;61(1):14-8. doi: 10.4315/0362-028x-61.1.14.

DOI:10.4315/0362-028x-61.1.14

PMID:9708246

Abstract

The SimPlate Total Plate Count (TPC) method, developed by IDEXX Laboratories, Inc., is designed to determine the most probable number of aerobic microorganisms in foods. The 24-h test was compared to the conventional plate count agar (PCA) method, the Petrifilm Aerobic Count plates, and the Redigel Total Count procedure for enumerating microflora in 751 food samples. Results using the SimPlate TPC method were highly correlated (r > or = 0.96) with results from other test methods. Slopes (0.96-0.97) were not significantly different from 1, and y intercepts (-0.03-0.08) were not different from O. The SimPlate has a high counting range (> 1600 most probable number per single dilution), thus requiring fewer dilutions of samples compared to other methods evaluated. Some foods, e.g., raw liver, wheat flour, and nuts, contain enzymes that gave false-positive reactions on SimPlates. Overall, however, the SimPlate TPC method is a suitable alternative to conventional PCA, Petrifilm, and Redigel methods for estimating populations of mesophilic aerobic microorganisms in a wide range of foods.

摘要

由IDEXX实验室公司开发的SimPlate总平板计数（TPC）方法，旨在测定食品中有氧微生物的最可能数量。将24小时检测法与传统平板计数琼脂（PCA）法、Petrifilm需氧菌计数平板法以及Redigel总计数法进行比较，以对751份食品样本中的微生物区系进行计数。使用SimPlate TPC方法得到的结果与其他检测方法的结果高度相关（r≥0.96）。斜率（0.96 - 0.97）与1无显著差异，y轴截距（-0.03 - 0.08）与0无差异。SimPlate具有较高的计数范围（每单倍稀释液中最可能数量>1600），因此与所评估的其他方法相比，需要对样本进行的稀释次数更少。一些食品，如生肝脏、小麦粉和坚果，含有会在SimPlate上产生假阳性反应的酶。然而，总体而言，SimPlate TPC方法是估计多种食品中嗜温性需氧微生物数量的一种适用于替代传统PCA、Petrifilm和Redigel方法的方法。