相互作用的RNA聚合酶II亚基hRPB11和hRPB3在成人组织中协同表达，并被阿霉素下调。

The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down-regulated by doxorubicin.

作者信息

Fanciulli M, Bruno T, Di Padova M, De Angelis R, Lovari S, Floridi A, Passananti C

机构信息

Cell Metabolism and Pharmacokinetics Laboratory, Regina Elena Cancer Institute, Rome, Italy.

出版信息

FEBS Lett. 1998 May 8;427(2):236-40. doi: 10.1016/s0014-5793(98)00431-1.

DOI:10.1016/s0014-5793(98)00431-1

Abstract

We previously isolated the human RPB11 cDNA, encoding the 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression of this subunit is modulated by doxorubicin. Using hRPB11 as bait in a yeast two-hybrid system, two cDNA variants encoding a second RNA polymerase II subunit, hRPB3, have now been isolated and characterized. These two hRPB3 mRNA species differed in 3' UTR region length, the longer transcript containing the AU-rich sequence motif that mediates mRNA degradation. Both hRPB11 and hRPB3 transcripts share a similar pattern of distribution in human adult tissues, with particularly high levels in both heart and skeletal muscle, and the expression of both is down-regulated by doxorubicin as found previously for the hRPB11 subunit. Taken together, these findings suggest that the interaction between hRPB3 and hRPB11 is fundamental for their function and that this heterodimer is involved in doxorubicin toxicity.

摘要

我们之前分离出了编码RNA聚合酶II 13.3 kDa亚基的人RPB11 cDNA，并证明该亚基的表达受阿霉素调节。在酵母双杂交系统中以hRPB11作为诱饵，现已分离并鉴定出两个编码第二个RNA聚合酶II亚基hRPB3的cDNA变体。这两种hRPB3 mRNA在3'UTR区域长度上有所不同，较长的转录本含有介导mRNA降解的富含AU的序列基序。hRPB11和hRPB3转录本在人类成人组织中具有相似的分布模式，在心脏和骨骼肌中含量特别高，并且正如之前发现的hRPB11亚基一样，两者的表达都被阿霉素下调。综上所述，这些发现表明hRPB3和hRPB11之间的相互作用对它们的功能至关重要，并且这种异二聚体参与了阿霉素毒性作用。