Schantz S P, Kolli V, Savage H E, Yu G, Shah J P, Harris D E, Katz A, Alfano R R, Huvos A G
Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Clin Cancer Res. 1998 May;4(5):1177-82.
Native cellular fluorescence (NCF) represents the innate capacity of tissues to absorb and emit light of a specified wavelength. The ability to define the relationship of in vivo NCF with biological characteristics of neoplastic disease may allow for an improved understanding of the clinical course of disease. Head and neck cancers from 35 patients were evaluated in vivo for NCF characteristics using a xenon lamp-based spectrometer coupled to a handheld fiberoptic probe. Spectral assessment was limited to lambda 450-nm emission characteristics, in which tissues were excited at various wavelengths, ranging from lambda 290 nm to lambda 415 nm, and the intensity of lambda 450 nm emission was recorded. Each cancer was subsequently biopsied and assessed for histological differentiation by a pathologist who was blinded to NCF analysis. Considerable variation in spectral characteristics between head and neck cancers was identified, which was determined, in part, by NCF characteristics of the normal mucosa from the same patient. Poorly differentiated tumors were more likely than well- or moderately differentiated tumors to have lower excitation maxima (P < 0.05 by ANOVA). Most significantly, the tumor differentiation status, as well as the probability of demonstrating recurrent disease, could also be related to the NCF characteristics of the patient's normal mucosa from the same site within the upper aerodigestive tract. NCF analysis may represent an effective tool to identify biological characteristics of head and neck tumors in vivo without the need for invasive biopsies. Results suggest the need to explore the determinants of NCF characteristics expressed by clinically normal mucosa.
天然细胞荧光（NCF）代表组织吸收和发射特定波长光的固有能力。明确体内NCF与肿瘤疾病生物学特征之间的关系，可能有助于更好地理解疾病的临床进程。使用与手持式光纤探头耦合的基于氙灯的光谱仪，对35例患者的头颈部癌进行了体内NCF特征评估。光谱评估仅限于450纳米发射特征，其中组织在290纳米至415纳米的不同波长下被激发，并记录450纳米发射的强度。随后对每个癌症进行活检，并由一位对NCF分析不知情的病理学家评估组织学分化情况。研究发现头颈部癌之间的光谱特征存在显著差异，部分差异由同一患者正常黏膜的NCF特征决定。低分化肿瘤比高分化或中分化肿瘤更有可能具有较低的激发最大值（方差分析，P < 0.05）。最显著的是，肿瘤分化状态以及出现复发疾病的可能性，也可能与上消化道同一部位患者正常黏膜的NCF特征有关。NCF分析可能是一种无需进行侵入性活检就能在体内识别头颈部肿瘤生物学特征的有效工具。结果表明有必要探索临床正常黏膜所表达的NCF特征的决定因素。
