Kubota T
School of Allied Health Sciences, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
J Med Dent Sci. 1997 Jun;44(2):37-44.
In this graduate school of Tokyo Medical and Dental University, I was given a somewhat vague but farsighted theme by a professor: "protein-DNA interaction." Within these simple words, I was given a free hand. Being curious about the etiology of connective tissue diseases, I began to study the biochemistry and pathophysiology of autoimmunity, especially the nature of anti-DNA antibodies that are the principal anti-nuclear antibodies observed in patients with systemic lupus erythematosus (SLE). My thesis was on the characterization of serum anti-DNA antibodies purified by a novel method of affinity column chromatography. Thereafter, I remained involved in this fascinating field. In spite of the rapid progress in molecular immunology, the etiology of any particular systemic autoimmune disorder remains elusive at this point. Here, works on a monoclonal anti-DNA antibody performed in the laboratories of Dr. B. D. Stollar (Tufts University, Boston), Dr. Y. Kanai (University of Tokyo), and in our laboratory will be reviewed along with related articles.
在东京医科齿科大学研究生院，一位教授给了我一个有些模糊但有远见的主题：“蛋白质 - DNA 相互作用”。在这简单的几个字范围内，我可以自由发挥。出于对结缔组织疾病病因的好奇，我开始研究自身免疫的生物化学和病理生理学，特别是抗 DNA 抗体的性质，这些抗体是系统性红斑狼疮（SLE）患者中观察到的主要抗核抗体。我的论文是关于用一种新型亲和柱色谱法纯化血清抗 DNA 抗体的特性研究。此后，我一直投身于这个迷人的领域。尽管分子免疫学取得了快速进展，但任何特定的系统性自身免疫性疾病的病因在目前仍然难以捉摸。在此，将回顾在 B. D. 斯托拉尔博士（波士顿塔夫茨大学）、金井洋博士（东京大学）的实验室以及我们实验室进行的关于单克隆抗 DNA 抗体的研究工作以及相关文章。
