Asahina I, Watanabe M, Sakurai N, Mori M, Enomoto S
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
J Med Dent Sci. 1997 Sep;44(3):63-70.
The purpose of this study was to evaluate a hydroxyapatite (HA)-collagen (Col)-bone morphogenetic protein (BMP) composite as an osteoinductive bone substitute. Partially-purified BMP from bovine bone was mechanically mixed with highly purified type I collagen from calf dermis and then subsequently mixed with pure synthetic hydroxyapatite granules or block. The HA-Col-BMP composite, or the HA-Col composite as a control, was implanted in a surgically-induced mandible bone defect (6 x 7 x 10 mm) in an adult Japanese monkey. The mandible was excised three months after implantation and studied histologically. The BMP-containing implant induced much more new bone than the control implant in all experimental animals of each group, with either HA granules or HA block. Newly formed bone was attached tightly to HA and infiltrated deeply into the pores of the HA of the BMP-containing implant, while fibrous tissue existed between the host bone and HA in the control implant. Thus, we conclude that an HA-Col-BMP composite could be a superior biomaterial for a bone substitute.
本研究的目的是评估羟基磷灰石（HA）-胶原蛋白（Col）-骨形态发生蛋白（BMP）复合材料作为一种具有骨诱导性的骨替代物。将从牛骨中部分纯化的BMP与从小牛真皮中高度纯化的I型胶原蛋白机械混合，然后再与纯合成羟基磷灰石颗粒或块状物混合。将HA-Col-BMP复合材料或作为对照的HA-Col复合材料植入成年日本猕猴手术造成的下颌骨缺损（6×7×10毫米）处。植入三个月后切除下颌骨并进行组织学研究。在每组的所有实验动物中，无论使用HA颗粒还是HA块状物，含BMP的植入物诱导生成的新骨都比对照植入物多得多。新形成的骨紧密附着于HA，并深入渗透到含BMP植入物的HA孔隙中，而对照植入物中宿主骨与HA之间存在纤维组织。因此，我们得出结论，HA-Col-BMP复合材料可能是一种用于骨替代的优质生物材料。
