Attanasio M, Gori A M, Giusti B, Pepe G, Comeglio P, Brunelli T, Prisco D, Abbate R, Gensini G F, Neri Serneri G G
Istituto di Clinica Medica Generale e Cardiologia, University of Florence, Italy.
Thromb Haemost. 1998 May;79(5):959-62.
In addition to its well-understood anticoagulant activity, heparin is known to modulate a variety of biological functions including immunologic responses. In order to investigate whether heparin influences the humoral immunity by interacting with cellular elements and affecting gene expression in blood circulating cells. we studied the effect of heparin on IL-1beta, IL-6 and TNFalpha mRNAs in human lipopolysaccharide-(LPS)- or interferon-gamma(IFN-gamma)-stimulated mononuclear cells. The study of mRNA was carried out by an initial PCR screening followed by a Northern blot quantitative analysis. Heparin (0.5 U/ml) turned out to inhibit all three cytokine gene expressions. The mRNA decrease was 37 +/- 6% for IL-1beta, 53 +/- 3% for IL-6 and 47 +/- 4% for TNFalpha with LPS stimulus. No differences could be observed in the inhibitory effect of heparin on IFNgamma-stimulated cells. This effect of heparin was confirmed in a subset of experiments performed on purified monocytes. These results suggest an important immunosuppressive effect of heparin on cell-mediated immune responses.
除了其广为人知的抗凝活性外，肝素还能调节多种生物学功能，包括免疫反应。为了研究肝素是否通过与细胞成分相互作用并影响血液循环细胞中的基因表达来影响体液免疫，我们研究了肝素对人脂多糖（LPS）或干扰素γ（IFN-γ）刺激的单核细胞中IL-1β、IL-6和TNFα mRNA的影响。mRNA的研究首先通过PCR筛选，然后进行Northern印迹定量分析。结果表明，肝素（0.5 U/ml）可抑制所有三种细胞因子基因的表达。在LPS刺激下，IL-1β的mRNA减少37±6%，IL-6减少53±3%，TNFα减少47±4%。未观察到肝素对IFNγ刺激细胞的抑制作用有差异。在对纯化单核细胞进行的一部分实验中证实了肝素的这种作用。这些结果表明肝素对细胞介导的免疫反应具有重要的免疫抑制作用。
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