Kirkpatrick U J, Adams R A, Lardi A, McCollum C N
Vascular Studies Unit, University Hospital of South Manchester, Manchester.
Br J Haematol. 1998 May;101(2):364-8. doi: 10.1046/j.1365-2141.1998.00689.x.
The technique involving filtration of diluted blood enables the separate analysis of the flow properties of different cell subpopulations. This study was designed to assess the changes occurring in the flow properties and function of blood cells in stored bank blood and salvaged blood compared to patient blood in a given clinical situation. We measured hydrogen peroxide production by neutrophils and the filterability, through 5 microm Nucleopore filters, of isolated red blood cells and of diluted blood. Samples were obtained from patients undergoing aortic surgery and blood intended for transfusion: either salvaged during surgery or stored bank blood. Both salvaged and bank blood were much less filterable than patient blood, with reduced deformability of both red and white blood cells. However, salvaged blood contained highly activated neutrophils with a prolonged transit time of the 'fast-flowing' cells in the analysis compared to bank blood. Bank blood contained significantly more particles which acted as pore-blockers. Cells in bank and salvaged blood therefore have markedly abnormal flow and biochemical properties compared to patient blood.
涉及稀释血液过滤的技术能够对不同细胞亚群的流动特性进行单独分析。本研究旨在评估在特定临床情况下，与患者血液相比，库存血和回收血中血细胞的流动特性和功能所发生的变化。我们测量了中性粒细胞产生过氧化氢的情况，以及分离出的红细胞和稀释血液通过5微米核孔滤膜的过滤性。样本取自接受主动脉手术的患者以及用于输血的血液：要么是手术中回收的血液，要么是库存血。回收血和库存血的可过滤性均远低于患者血液，红细胞和白细胞的变形性均降低。然而，与库存血相比，回收血中含有高度活化的中性粒细胞，在分析中“快速流动”细胞的通过时间延长。库存血中含有明显更多充当孔堵塞物的颗粒。因此，与患者血液相比，库存血和回收血中的细胞具有明显异常的流动和生化特性。
