结核分枝杆菌分离株噬菌体分型所用方法的改进。
Modification of methods used in bacteriophage typing of Mycobacterium tuberculosis isolates.
作者信息
Jones W D, Greenberg J
出版信息
J Clin Microbiol. 1978 May;7(5):467-9. doi: 10.1128/jcm.7.5.467-469.1978.
Abstract
A procedure in which soft agar overlays were used in bacteriophage-typing Mycobacterium tuberculosis is presented. This safer method uses commercially available media, whereas media presently used must be prepared in the laboratory. Single plaque isolations of the phage BG-1 specifying phage type A and B of M. tuberculosis were readily made by using the modified procedures. This purification and the use of prototype strain Myc 1415 as the indicator host strain have significantly enhanced the ability to discriminate among strains of phage types A and B.
摘要
本文介绍了一种在结核分枝杆菌噬菌体分型中使用软琼脂覆盖层的方法。这种更安全的方法使用市售培养基，而目前使用的培养基必须在实验室制备。通过使用改进的程序，可以轻松地对指定结核分枝杆菌A和B型噬菌体的BG-1噬菌体进行单噬菌斑分离。这种纯化方法以及使用原型菌株Myc 1415作为指示宿主菌株，显著提高了区分A和B型噬菌体菌株的能力。
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