Pescovitz M D, Book B K, Aasted B, Dominguez J, Ezquerra A, Trebichavsky I, Novikov B, Valpotic I, Nielsen J, Arn S, Sachs D H, Lunney J K, Boyd P C, Walker J, Lee R, Lackovic G, Kirkham P, Parkhouse R M, Saalmüller A
Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis 46202, USA.
Vet Immunol Immunopathol. 1998 Jan 30;60(3-4):261-8. doi: 10.1016/s0165-2427(97)00102-5.
Among the 57 monoclonal antibodies (mAb) analyzed within the T-cell group from the Second Swine CD Workshop, six mAb fell within clusters T10 and T11 (No. 088, STH164; No. 148, FY1A3; No. 149, FY2C1; No. 150, FY1H2; No. 151, FY2A11; No. 169, BB23-8E6). The mAb within these two groups gave a similar appearance on flow cytometry and stained all peripheral blood T-cells as defined by CD4 and wCD8 staining. All six mAb precipitated a 24 kDa protein. On the basis of inhibition analyses performed as part of the workshop and from published data, the mAb define at least three epitopes. There is only minimal stimulation of resting peripheral lymphocytes, but four of the mAb produce strong stimulation in the presence of PMA. With the exception of STH164, all have been shown to react with CD3 epsilon-transfected COS cells. The new mAb, therefore, react with three epitopes on porcine CD3 epsilon designated CD3a (BB23-8E6, FY2A11), CD3b (FY1A3, FY2C1), and CD3c (FY1H2). mAb STH164 appears to be reactive with another epitope, however, since its reactivity with CD3 has not been confirmed it is designated as wCD3.
在第二届猪CD研讨会的T细胞组分析的57种单克隆抗体（mAb）中，有6种mAb属于T10和T11簇（编号088，STH164；编号148，FY1A3；编号149，FY2C1；编号150，FY1H2；编号151，FY2A11；编号169，BB23 - 8E6）。这两组内的mAb在流式细胞仪上呈现相似外观，并能对通过CD4和wCD8染色定义的所有外周血T细胞进行染色。所有6种mAb都沉淀出一种24 kDa的蛋白质。根据研讨会期间进行的抑制分析以及已发表的数据，这些mAb定义了至少三个表位。对静息外周淋巴细胞只有最小程度的刺激，但其中4种mAb在有佛波酯（PMA）存在时能产生强烈刺激。除了STH164外，所有mAb都已被证明能与CD3ε转染的COS细胞发生反应。因此，这些新的mAb与猪CD3ε上的三个表位发生反应，分别命名为CD3a（BB23 - 8E6，FY2A11）、CD3b（FY1A3，FY2C1）和CD3c（FY1H2）。然而，mAb STH164似乎与另一个表位发生反应，由于其与CD3的反应性尚未得到证实，故将其命名为wCD3。
