γ/δT细胞限制性抗原WC1的分析
Analysis of the gamma/delta T cell restricted antigen WC1.
作者信息
Crocker G, Sopp P, Parsons K, Davis W C, Howard C J
机构信息
AFRC Institute for Animal Health, Compton, Newbury, UK.
出版信息
Vet Immunol Immunopathol. 1993 Nov;39(1-3):137-44. doi: 10.1016/0165-2427(93)90174-3.
The nine antibodies (mAbs) which clustered with the standard WC1 mAb CC15 in temporary cluster TC22 were analysed by immunoprecipitation, flow cytometry and immunohistology. Two forms of the molecule, with molecular weights of 205 and 215 kDa, were identified by immunoprecipitation and SDS-PAGE, the expression of which correlated with subclusters generated by flow cytometric analysis. Immunohistological data, however, did not reveal any tissue specific distribution. We propose the existence of at least two isoforms of WC1 which may prove to identify subpopulations of CD2- CD4- CD8- gamma/delta T cells.
通过免疫沉淀、流式细胞术和免疫组织学分析了在临时簇TC22中与标准WC1单克隆抗体CC15聚集的九种单克隆抗体（mAb）。通过免疫沉淀和SDS-PAGE鉴定出该分子的两种形式，分子量分别为205 kDa和215 kDa，其表达与流式细胞术分析产生的亚群相关。然而，免疫组织学数据未显示任何组织特异性分布。我们提出存在至少两种WC1同工型，这可能有助于识别CD2-CD4-CD8-γ/δT细胞的亚群。
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