专性甲基营养菌鞭毛甲基杆菌的精细遗传图谱。
Refined genetic map of the obligate methylotroph Methylobacillus flagellatum.
作者信息
Serebrijski I, Gomelsky M, Bashkirova E, Chistoserdov A, Tsygankov Y D
机构信息
Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
出版信息
Mol Gen Genet. 1998 Apr;258(1-2):133-8. doi: 10.1007/s004380050715.
We present a refined genetic map of the obligate methylotroph Methylobacillus flagellatum. New, Hfr (high-frequency-of-transfer) donors, and pulsed-field gel electrophoresis, were used to determine that M. flagellatum contains one approximately 3.1-Mb circular chromosome, and no plasmids. A correlation between time-of-entry units and DNA length was established. Using in vivo and in vitro cloning, and sequencing, a number of new genetic markers were identified and mapped; in addition, the nature of some of the previously mapped markers was elucidated.
我们展示了专性甲基营养菌鞭毛甲基杆菌的精细遗传图谱。利用新的高频转移（Hfr）供体和脉冲场凝胶电泳，确定鞭毛甲基杆菌含有一条约3.1 Mb的环状染色体，且无质粒。建立了进入时间单位与DNA长度之间的相关性。通过体内和体外克隆及测序，鉴定并定位了许多新的遗传标记；此外，还阐明了一些先前定位标记的性质。
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