Kletsova L V, Tsygankov Y D
Institute of Genetics, Moscow, USSR.
Arch Microbiol. 1990;153(2):139-45. doi: 10.1007/BF00247811.
Homologous matings with plasmids R68.45 and pULB113, and also with Hfr type donor were employed for mapping pgi and gpd genes involved in C-1 metabolism in the obligate methylotroph Methylobacillus flagellatum. A preliminary map of the late chromosomal region was constructed on the basis of these experimental results. The C-1 markers were linked to methionine and leucine auxotrophy and nalidixic acid resistance markers. The phenomenon of retrotransfer, or shuttle transfer of chromosomal markers by Inc P1 plasmids, revealed earlier, was demonstrated for M. flagellatum.
利用质粒R68.45和pULB113以及Hfr型供体进行同源交配，对专性甲基营养菌鞭毛甲基杆菌中参与C-1代谢的pgi和gpd基因进行定位。基于这些实验结果构建了晚期染色体区域的初步图谱。C-1标记与蛋氨酸和亮氨酸营养缺陷型以及萘啶酸抗性标记相关联。之前发现的Inc P1质粒对染色体标记的反向转移或穿梭转移现象在鞭毛甲基杆菌中得到了证实。
